| Author | Moreira, Thayse Batista | |
| Author | Silvestrini, Marina Malheiros Araujo | |
| Author | Magalhães, Ana Luiza de Freitas | |
| Author | Rangel, Kerstin Kapp | |
| Author | Costa, Alvaro Percinio | |
| Author | Gomes, Matheus Souza | |
| Author | Amaral, Laurence Rodrigues do | |
| Author | Martins Filho, Olindo Assis | |
| Author | Salles, Paulo Guilherme de Oliveira | |
| Author | Braga, Leticia Conceição | |
| Author | Carvalho, Andrea Teixeira de | |
| Access date | 2025-04-14T17:23:17Z | |
| Available date | 2025-04-14T17:23:17Z | |
| Document date | 2025 | |
| Citation | MOREIRA, Thayse Batista et al. Neutrophil-and Endothelial Cell-Derived Extracellular Microvesicles Are Promising Putative Biomarkers for Breast Cancer Diagnosis. Biomedicines, v. 13, n. 3, 587, 2025. | en_US |
| ISSN | 2227-9059 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/69630 | |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | en_US |
| Title | Neutrophil-and Endothelial Cell-Derived Extracellular Microvesicles Are Promising Putative Biomarkers for Breast Cancer Diagnosis. | en_US |
| Type | Article | en_US |
| DOI | 10.3390/biomedicines13030587 | |
| Abstract | Introduction: Breast cancer (BC) is a disease that affects about 2.2 million people worldwide. The prognosis and treatment of these patients depend on clinical and histopathologic staging, in which more aggressive cancers need a less conservative therapeutic approach. Previous studies showed that patients with BC have an increased frequency of systemic microvesicles (MVs) that are associated with invasion, progression, and metastasis, which can be used in liquid biopsy to predict the therapeutic response in individualized treatment. Objective: This study proposes the development of a minimally invasive BC diagnostic panel and follow-up biomarkers as a complementary method to screen patients. Methods: The quantification of circulating MVs in 48 healthy women and 100 BC patients who attended the Mario Penna Institute between 2019 and 2022 was performed by flow cytometry. In addition, the MVs of BC patients were analyzed before treatment and 6, 12, and 24 months post-treatment. Machine learning approaches were employed to determine the performance of MVs to identify BC and to propose BC classifier algorithms. Results: Patients with BC had more neutrophil- and endothelial cell-derived MVs than controls before treatment. After treatment, all MV populations were decreased compared to pre-treatment, but leukocyte- and erythrocyte-derived MVs were increased at 12 months after treatment, before decreasing again at 24 months. Conclusions: Performance analyses and machine learning approaches pointed out that MVs from neutrophils and endothelial cells are the best candidates for BC diagnostic biomarkers. Neutrophil- and endothelial cell-derived MVs are putative candidates for BC biomarkers to be employed as screening tests for BC diagnosis. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Instituto Mario Penna. Hospital Luxemburgo. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Instituto Mario Penna. Hospital Luxemburgo. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Instituto Mario Penna. Hospital Luxemburgo. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Universidade Federal de Uberlândia. Laboratório de Bioinformática e Analise Molecular. Patos de Minas, MG, Brasil. | en_US |
| Affilliation | Universidade Federal de Uberlândia. Laboratório de Bioinformática e Analise Molecular. Patos de Minas, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Instituto Mario Penna. Hospital Luxemburgo. Belo Horizonte, MG, Brasil. / Instituto Mario Penna. Hospital Luxemburgo. Laboratório de Anatomia Patológica. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Instituto Mario Penna. Instituto de Ensino, Pesquisa e Inovação. Laboratório de Pesquisa Translacional em Oncologia. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | en_US |
| Subject | Breast cancer | en_US |
| Subject | Liquid biopsy | en_US |
| Subject | Extracellular microvesicles | en_US |
| Subject | Flow cytometry | en_US |
| Subject | Biomarkers | en_US |
