| Author | Souza, Bárbara da Silva de | |
| Author | Lima, Letícia Ferreira | |
| Author | Galardo, Allan Kardec Ribeiro | |
| Author | Corbel, Vincent | |
| Author | Lima, José Bento Pereira | |
| Author | Martins Júnior, Ademir de Jesus | |
| Access date | 2025-04-25T15:59:23Z | |
| Available date | 2025-04-25T15:59:23Z | |
| Document date | 2023 | |
| Citation | SOUZA, Bárbara da Silva de et al. Genetic structure and kdr mutations in Aedes aegypti populations along a road crossing the Amazon Forest in Amapá State, Brazil. Scientific Reports, v. 13, n. 17167, p. 1-11, 11 Oct. 2023. | |
| ISSN | 2045-2322 | |
| URI | https://www.arca.fiocruz.br/handle/icict/69894 | |
| Description | Produção científica do Laboratório de Biologia, Controle e Vigilância de Insetos Vetores. | pt_BR |
| Sponsorship | This study was funded by CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Grant No. 316730/2021-1); FAPERJ (Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro—Jovem Cientista do Nosso Estado, Grant No. 26/202.795/2019); INCT-EM (Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular); PPSUS (Programa de Pesquisa para o SUS, Grant No. 250.203.100/2016. This study was partially supported by the Coordination for the Improvement of Higher Education Personnel (CAPES—Finance Code 001), and by the European Union (HORIZON-MSCA-2021-SE-01 INOVEC project, Grant No 101086257). | |
| Language | eng | en_US |
| Publisher | Nature Research | |
| Rights | open access | |
| Title | Genetic structure and kdr mutations in Aedes aegypti populations along a road crossing the Amazon Forest in Amapá State, Brazil | en_US |
| Type | Article | |
| DOI | 10.1038/s41598-023-44430-x | |
| Abstract | Insecticide resistance in Aedes aegypti poses a significant threat to disease control. One form of resistance, caused by kdr mutations in the NaV gene, hinders vector control efforts in Brazil. Despite genetic differences typically accumulating among isolated populations, this mosquito can actively and passively disperse through human transportation. Our study investigated the genetic structure and spread of kdr mutations in Ae. aegypti populations across six localities in Amapá State, Brazil, within the Amazonian Forest. Using 12 microsatellite loci and qPCR methods, we assessed genetic structure and identified three common kdr mutations (V410L, V1016I, and F1534C). High prevalence of kdr alleles was observed in all localities, indicating widespread distribution in Amapá State. Microsatellite analysis revealed differentiation among mosquito populations, dividing them into two distinct clusters supported by Bayesian and DAPC analyses. Oiapoque, located along the northern border with French Guiana, exhibited the highest kdr frequencies and genetic differentiation compared to other localities. Our findings suggest genetic structure in Ae. aegypti populations in Amapá State, with some passive gene flow between clusters. The study underscores the need for continuous surveillance of Ae. aegypti populations to monitor the spread of insecticide resistance and inform effective vector control strategies. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia, Controle e Vigilância de Insetos Vetores. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de Pós-Graduação em Biologia Parasitária. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências Matemáticas e da Natureza. Instituto de Química. Departamento de Bioquímica. Laboratório de Bioinformática. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Governo do Estado do Amapá. Instituto de Pesquisas Científicas e Tecnológicas do Estado do Amapá. Macapá, AP, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia, Controle e Vigilância de Insetos Vetores. Rio de Janeiro, RJ, Brasil / Université de Montpellier. Institut de Recherche Pour le Développement. Centre National de la Recherche Scientifique. Maladies Infectieuses et Vecteurs: Écologie, Génétique, Évolution et Contrôle. Montpellier, France. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia, Controle e Vigilância de Insetos Vetores. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia, Controle e Vigilância de Insetos Vetores. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Bioquímica Médica Leopoldo de Meis. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil. | |
| Subject | Entomology | en_US |
| Subject | Genetic markers | en_US |
| Subject | Population genetics | en_US |
| DeCS | Entomologia | pt_BR |
| DeCS | Marcadores Genéticos | pt_BR |
| DeCS | Genética Populacional | pt_BR |
| e-ISSN | 2045-2322 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
| xmlui.metadata.dc.subject.ods | 09 Indústria, inovação e infraestrutura | |
| xmlui.metadata.dc.subject.ods | 11 Cidades e comunidades sustentáveis | |
| xmlui.metadata.dc.subject.ods | 17 Parcerias e meios de implementação | |
