| Author | Gonçalves, Ana Carolina Aparecida | |
| Author | Conzentino, Marcelo dos Santos | |
| Author | Barbosa, Altina Bruna de Souza | |
| Author | Doline, Fernando Rodrigo | |
| Author | Nilsson, Mariana Guimarães | |
| Author | Silva, Aristeu Vieira da | |
| Author | Kmetiuk, Louise Bach | |
| Author | Biondo, Alexander Welker | |
| Author | Huergo, Luciano Fernandes | |
| Access date | 2025-05-04T21:28:05Z | |
| Available date | 2025-05-04T21:28:05Z | |
| Document date | 2024 | |
| Citation | GONÇALVES, Ana Carolina Aparecida et al. Ultrafast and high-throughput immunoassay assay to detect antiSARS-CoV-2 IgG antibodies in dogs and cats. Brazilian Journal of Microbiology, v. 55, p. 4183–4189, 2024. | en_US |
| ISSN | 1678-4405 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/70119 | |
| Sponsorship | Present research was funded through the Brazilian National Council for Scientific and Technological Development (CNPq) (2020-1/402341), and Coordination for the Improvement of Higher Education Personnel (CAPES). | en_US |
| Language | eng | en_US |
| Publisher | Sociedade Brasileira de Microbiologia | en_US |
| Rights | closed access | en_US |
| Subject in Portuguese | COVID-19 | en_US |
| Subject in Portuguese | Imunoensaio magnético | en_US |
| Subject in Portuguese | Cães | en_US |
| Subject in Portuguese | SARS-CoV-2 | en_US |
| Subject in Portuguese | Gatos | en_US |
| Title | Ultrafast and high-throughput immunoassay assay to detect anti-SARS-CoV-2 IgG antibodies in dogs and cats | en_US |
| Type | Article | en_US |
| DOI | 10.1007/s42770-024-01518-4 | |
| Abstract | The fact that SARS-CoV-2 has reportedly infected companion, livestock and wildlife animals may constitute a significant risk for virus reservoirs, ground for emerging variants and potential for novel reverse zoonosis. Hence, SARS-CoV-2 surveillance in animal species is crucial to prevent emerging variants which may spread to humans. The present study aimed to develop a simple, high-throughput and ultrafast magnetic bead immunoassay to detect anti-SARS-CoV-2 nucleocapsid and spike reactive IgG antibodies in dog and cat serum samples. The assays were validated using serum from eleven dogs and cats which had SARS-CoV-2 infections confirmed by real-time RT-PCR. The negative cohort consisted
of pre-pandemic dog and cat samples. The assays performed at 73–82% sensitivity and 97.5–98% specificity for dogs and 71% sensitivity and 92–94% specificity for cats. The lower assay specificity for cats is explained by the fact that cat pre-pandemic sera showed high levels of cross-reactive with SARS-CoV-2 Nucleocapsid and Spike, supporting that these animals have been exposed to other coronavirus sharing structural similarities with SARS-CoV-2. These assays described in this work are now being used for SARS-CoV-2 surveillance and research purposes. | en_US |
| Affilliation | Federal University of Paraná. Seashore Campus Setor Litoral. Matinhos, PR, Brazil. | en_US |
| Affilliation | Federal University of Paraná. Seashore Campus Setor Litoral. Matinhos, PR, Brazil. | en_US |
| Affilliation | Federal University of Paraná. Graduate College of Cellular and Molecular Biology. Curitiba, PR, Brazil. | en_US |
| Affilliation | Federal University of Paraná. Graduate College of Cellular and Molecular Biology. Curitiba, PR, Brazil. | en_US |
| Affilliation | State University of Feira de Santana. Zoonosis and Public Health Research Group. Feira de Santana, BA, Brazil. | en_US |
| Affilliation | State University of Feira de Santana. Zoonosis and Public Health Research Group. Feira de Santana, BA, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Federal University of Paraná. Graduate College of Cellular and Molecular Biology. Curitiba, PR, Brazil. | en_US |
| Affilliation | Federal University of Paraná. Seashore Campus Setor Litoral. Matinhos, PR, Brazil. | en_US |
| Subject | COVID-19 | en_US |
| Subject | Magnetic immunoassay | en_US |
| Subject | Dogs | en_US |
| Subject | SARS-CoV-2 | en_US |
| Subject | Cats | en_US |
| DeCS | SARS-CoV-2 | en_US |
| DeCS | COVID-19 | en_US |
| DeCS | Cães | en_US |
| Embargo date | 2054 | |
