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| Autor | Nelson, Elisabeth | |
| Autor | Pereira, Thiago Nunes | |
| Autor | Ribeiro, Erica Milena de Castro | |
| Autor | Silva, Bianca Daoud Mafra E | |
| Autor | Camillo, Carolina | |
| Autor | Costa, Thiago Rodrigues da | |
| Autor | Teixeira, Mauro M | |
| Autor | Ko, Albert I | |
| Autor | Cummings, Derek A T | |
| Autor | Moreira, Luciano Andrade | |
| Fecha de acceso | 2025-05-06T19:47:04Z | |
| Fecha de disponibilización | 2025-05-06T19:47:04Z | |
| Fecha de publicación | 2025 | |
| Referencia | NELSON, Elisabeth et al. Comparison of oviposition and adult trapping to monitor wMel introgression for Wolbachia-based vector control. BioRxiv, 2025, 10.642347, 2025. | en_US |
| ISSN | 2692-8205 | |
| URI | https://www.arca.fiocruz.br/handle/icict/70207 | |
| Idioma | eng | en_US |
| Editor | Cold Spring Harbor Laboratory | en_US |
| Derechos de autor | open access | en_US |
| Palabras clave en Portugués | Wolbachia | en_US |
| Palabras clave en Portugués | Aedes aegypti | en_US |
| Título | Comparison of oviposition and adult trapping to monitor wMel introgression for Wolbachia-based vector control. | en_US |
| Tipo del documento | Preprint | en_US |
| DOI | 10.1101/2025.03.10.642347 | |
| Resumen en Inglés | Wolbachia introgression into Aedes aegypti mosquito populations has been shown to be effective in preventing dengue and is being evaluated for WHO prequalification. Monitoring the long-term introgression of Wolbachia (wMel)-positive Aedes aegypti mosquitoes, however, requires labor-intensive and costly BG-Sentinel traps (BG-traps). More affordable alternatives, such as using oviposition traps (ovitraps), have not been fully evaluated. Ae. aegypti eggs and adults were collected from 124 ovitraps and 237 BG-traps, respectively, across 12 clusters in Belo Horizonte, Brazil from March to May 2023 as part of the EVITA Dengue trial. We used a qPCR assay to detect wMel in a sample of L3-L4 stage larvae (up to 29) that were reared from eggs in ovitraps and adults from BG-traps (up to 10 per BG-trap). We used mixed effects models to compare estimates of cluster-level wMel introgression from ovitrap and BG-trap data over time. Among 3,675 larvae reared from ovitraps, wMel prevalence was 0.50 (95% CI: 0.48-0.51). Among 1,244 adult Ae. aegypti tested from BG-traps, wMel prevalence was 0.45 (95% CI: 0.42-0.48). Cluster-level wMel introgression in larvae and adults was highly correlated (Spearman's r = 0.70, p = 6.71e-06). Multivariate analysis found that ovitrap estimates of introgression were associated with BG-trap estimates in the same month when models incorporated the previous month's ovitrap wMel-positive count, the proportion of wMel in ovitraps in the current and previous month, and Ae. aegypti abundance. Leveraging this model, predicted wMel introgression from ovitrap data were highly correlated with observed introgression from BG-trap data (rs,counts=0.98, p=1.53e-14; rs,prevalences=0.82, p=0.11e-05) and provided greater precision than crude ovitrap-based estimates. These findings indicate that ovitrap-based monitoring represents a low cost, more efficient approach to evaluating introgression as the Wolbachia-based interventions are scaled up and implemented broadly in high burden regions for dengue and other arboviral diseases. | en_US |
| Afiliación | Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, USA. | en_US |
| Afiliación | Fundação Oswaldo Cruz. Instituto Rene Rachou. Programa Mundial de Mosquitos. Belo Horizonte, MG, Brasil. | en_US |
| Afiliación | Fundação Oswaldo Cruz. Instituto Rene Rachou. Programa Mundial de Mosquitos. Belo Horizonte, MG, Brasil. | en_US |
| Afiliación | Fundação Oswaldo Cruz. Instituto Rene Rachou. Programa Mundial de Mosquitos. Belo Horizonte, MG, Brasil. | en_US |
| Afiliación | Fundação Oswaldo Cruz. Instituto Rene Rachou. Programa Mundial de Mosquitos. Belo Horizonte, MG, Brasil. | en_US |
| Afiliación | Instituto Oswaldo Cruz. Programa Mundial de Mosquitos. Rio de Janeiro, RJ, Brasil. | en_US |
| Afiliación | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil. | en_US |
| Afiliación | Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, USA. / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Afiliación | Emerging Pathogens Institute and Department of Biology. University of Florida. Gainesville, USA. / Departments of Epidemiology and Biomedical Engineering. Johns Hopkins University. Baltimore, USA. | en_US |
| Afiliación | Fundação Oswaldo Cruz. Instituto Rene Rachou. Programa Mundial de Mosquitos. Belo Horizonte, MG, Brasil. | en_US |
| Palavras clave en Inglês | Wolbachia | en_US |
| Palavras clave en Inglês | Aedes aegypti | en_US |
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