| Author | Bafica, Andre Luiz Barbosa | |
| Author | Santiago, Helton da Costa | |
| Author | Goldszmid, Romina | |
| Author | Ropert, Catherine | |
| Author | Gazzinelli, Ricardo Tostes | |
| Author | Sher, Alan | |
| Access date | 2013-10-09T18:00:24Z | |
| Available date | 2013-10-09T18:00:24Z | |
| Document date | 2006 | |
| Citation | BAFICA, Andre et al. Cutting edge: TLR9 and TLR2 signaling together account for MyD88-dependent control of parasitemia in Trypanosoma cruzi infection. J Immunol. 2006 Sep 15;177(6):3515-9. | pt_BR |
| ISSN | 0022-1767 | |
| URI | https://www.arca.fiocruz.br/handle/icict/7103 | |
| Language | eng | pt_BR |
| Publisher | American Association of Immunologists | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Cutting edge: TLR9 and TLR2 signaling together account for MyD88-dependent control of parasitemia in Trypanosoma cruzi infection | pt_BR |
| Type | Article | pt_BR |
| Abstract | Activation of innate immune cells by Trypanosoma cruzi-derived molecules such as GPI anchors and DNA induces proinflammatory cytokine production and host defense mechanisms. In this study, we demonstrate that DNA from T. cruzi stimulates cytokine production by APCs in a TLR9-dependent manner and synergizes with parasite-derived GPI anchor, a TLR2 agonist, in the induction of cytokines by macrophages. Compared with wild-type animals, T. cruzi-infected Tlr9(-/-) mice displayed elevated parasitemia and decreased survival. Strikingly, infected Tlr2(-/-)Tlr9(-/-) mice developed a parasitemia equivalent to animals lacking MyD88, an essential signaling molecule for most TLR, but did not show the acute mortality displayed by MyD88(-/-) animals. The enhanced susceptibility of Tlr9(-/-) and Tlr2(-/-)Tlr9(-/-) mice was associated with decreased in vivo IL-12/IFN-gamma responses. Our results reveal that TLR2 and TLR9 cooperate in the control of parasite replication and that TLR9 has a primary role in the MyD88-dependent induction of IL-12/IFN-gamma synthesis during infection with T. cruzi. | pt_BR |
| Affilliation | National Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Parasitic Diseases. Immunobiology Section. Bethesda, USA | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | National Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Parasitic Diseases. Immunobiology Section. Bethesda, USA | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Imunopatologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Imunopatologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | National Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Parasitic Diseases. Immunobiology Section. Bethesda, USA | pt_BR |
| Subject | Adaptor proteins | pt_BR |
| Subject | Signal Transducing/physiology | pt_BR |
| Subject | Antigen-Presenting Cells/immunology | pt_BR |
| Subject | Chagas Disease/immunology | pt_BR |
| Subject | Interferon-gamma/metabolism | pt_BR |
| Subject | Toll-Like Receptor 2/genetics | pt_BR |
