| Author | Disch, Jolande | |
| Author | Caligiorne, Rachel Basques | |
| Author | Maciel, Fabiana | |
| Author | Oliveira, Maria Cláudia de | |
| Author | Orsini, Marcela | |
| Author | Dias Neto, Emmanuel | |
| Author | Rabello, Ana Lúcia Teles | |
| Access date | 2013-10-25T14:24:43Z | |
| Available date | 2013-10-25T14:24:43Z | |
| Document date | 2006 | |
| Citation | DISCH, Jolande et al. Single-step duplex kDNA-PCR for detection of Leishmania donovani complex in human peripheral blood samples. Diagn Microbiol Infect Dis. 2006 Dec;56(4):395-400 | pt_BR |
| ISSN | 0732-8893 | |
| URI | https://www.arca.fiocruz.br/handle/icict/7208 | |
| Language | eng | pt_BR |
| Publisher | Elsevier Biomedical | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Single-step duplex kDNA-PCR for detection of Leishmania donovani complex in human peripheral blood samples. | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.diagmicrobio.2006.07.002 | |
| Abstract | A duplex polymerase chain reaction (PCR) assay was performed using a sense Leishmania genus-specific oligonucleotides to amplify the conserved region of 120 bp of minicircle kDNA and an additional antisense Leishmania donovani oligonucleotide that amplifies a complex-specific fragment of 90 bp. All 12 tested reference isolates of the L. donovani complex yielded a complex-specific amplification product of 90 bp concurrently with a genus-specific 120-bp product. All 17 tested reference isolates pertaining to other complexes presented only the genus-specific 120-bp product. Peripheral blood samples of twenty patients with visceral leishmaniasis positive by the genus-specific PCR presented both fragments with the duplex assay. This duplex PCR can be applied as a 1-step diagnostic tool where discrimination of L. donovani complex species is relevant for clinical and epidemiologic purposes. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Pesquisas Clınicas. Belo Horizonte-MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Pesquisas Clınicas. Belo Horizonte-MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Pesquisas Clınicas. Belo Horizonte-MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Pesquisas Clınicas. Belo Horizonte-MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Clınica Medica. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Faculdade de Medicina Universidade de Sao Paulo. Instituto de Psiquiatria. Laboratorio de Neurociencias. Sao Paulo, SP, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Pesquisas Clınicas. Belo Horizonte-MG, Brazil | pt_BR |
| Subject | Visceral leishmaniasis | pt_BR |
| Subject | Diagnosis | pt_BR |
| Subject | PCR | pt_BR |
| Subject | Minicircle kDNA | pt_BR |
