| Author | Prates, Deboraci Brito | |
| Author | Santos, Théo de Araújo | |
| Author | Luz, Nívea Farias | |
| Author | Andrade, Bruno de Bezerril | |
| Author | Costa, Jaqueline França | |
| Author | Afonso, Lilian Maria | |
| Author | Clarêncio, Jorge | |
| Author | Miranda, José Carlos | |
| Author | Bozza, Patrícia T. | |
| Author | Reis, George A. dos | |
| Author | Brodskyn, Claudia Ida | |
| Author | Barral Netto, Manoel | |
| Author | Borges, Valeria de Matos | |
| Author | Barral, Aldina Maria Prado | |
| Access date | 2014-02-17T19:36:00Z | |
| Available date | 2014-02-17T19:36:00Z | |
| Document date | 2011 | |
| Citation | PRATES, D. B. et al. Lutzomyia longipalpis saliva drives apoptosis and enhances parasite burden in neutrophils. Journal of Leukocyte Biology, v. 90, n. 3, p.575-582, 2011. | pt_BR |
| ISSN | 1938-3673 | |
| URI | https://www.arca.fiocruz.br/handle/icict/7316 | |
| Language | eng | pt_BR |
| Publisher | Society for Leukocyte Biology | pt_BR |
| Rights | open access | pt_BR |
| Title | Lutzomyia longipalpis saliva drives apoptosis and enhances parasite burden in neutrophils | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1189/jlb.0211105 | |
| Abstract | Neutrophils are considered the host's first line of defense against infections and have been implicated in the immunopathogenesis of Leishmaniasis. Leishmania parasites are inoculated alongside vectors' saliva, which is a rich source of pharmacologically active substances that interfere with host immune response. In the present study, we tested the hypothesis that salivary components from Lutzomyia longipalpis, an important vector of visceral Leishmaniasis, enhance neutrophil apoptosis. Murine inflammatory peritoneal neutrophils cultured in the presence of SGS presented increased surface expression of FasL and underwent caspase-dependent and FasL-mediated apoptosis. This proapoptosis effect of SGS on neutrophils was abrogated by pretreatment with protease as well as preincubation with antisaliva antibodies. Furthermore, in the presence of Leishmania chagasi, SGS also increased apoptosis on neutrophils and increased PGE(2) release and decreased ROS production by neutrophils, while enhancing parasite viability inside these cells. The increased parasite burden was abrogated by treatment with z-VAD, a pan caspase inhibitor, and NS-398, a COX-2 inhibitor. In the presence of SGS, Leishmania-infected neutrophils produced higher levels of MCP-1 and attracted a high number of macrophages by chemotaxis in vitro assays. Both of these events were abrogated by pretreatment of neutrophils with bindarit, an inhibitor of CCL2/MCP-1 expression. Taken together, our data support the hypothesis that vector salivary proteins trigger caspase-dependent and FasL-mediated apoptosis, thereby favoring Leishmania survival inside neutrophils, which may represent an important mechanism for the establishment of Leishmania infection. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil | pt_BR |
| Affilliation | National Institute of Allergy and Infectious Diseases. National Institutes of Health. Laboratory of Parasitic Diseases. Besthesda, MD, USA | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia (iii-INCT). Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia (iii-INCT). Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia (iii-INCT). Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia (iii-INCT). Salvador, BA, Brasil | pt_BR |
| DeCS | Apoptose | pt_BR |
| DeCS | Leishmaniose/imunologia | pt_BR |
| DeCS | Neutrófilos/patologia | pt_BR |
| DeCS | Neutrófilos/parasitologia | pt_BR |
| DeCS | Psychodidae/imunologia | pt_BR |
| DeCS | Saliva/imunologia | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Caspases/metabolismo | pt_BR |
| DeCS | Quimiotaxia | pt_BR |
| DeCS | Quimiocina CCL2/metabolismo | pt_BR |
| DeCS | Feminino | pt_BR |
| DeCS | Proteína Ligante Fas/metabolismo | pt_BR |
| DeCS | Interações Hospedeiro-Parasita | pt_BR |
| DeCS | Immunoblotting | pt_BR |
| DeCS | Leishmania | pt_BR |
| DeCS | Leishmaniose/parasitologia | pt_BR |
| DeCS | Macrófagos/imunologia | pt_BR |
| DeCS | Masculino | pt_BR |
| DeCS | Camundongos | pt_BR |
| DeCS | Camundongos Endogâmicos C57BL | pt_BR |
| DeCS | Neutrófilos/imunologia | pt_BR |
| DeCS | Psychodidae/parasitologia | pt_BR |
| DeCS | Espécies de Oxigênio Reativas/metabolismo | pt_BR |
| DeCS | Saliva/química | pt_BR |
| DeCS | Saliva/parasitologia | pt_BR |
| DeCS | Glândulas Salivares/citologia | pt_BR |
| DeCS | Glândulas Salivares/imunologia | pt_BR |
| DeCS | Glândulas Salivares/parasitologia | pt_BR |
