| Author | Costa, Míriam Maria | |
| Author | Penido, Marcus Luiz de Oliveira | |
| Author | Gazzinelli, Ricardo Tostes | |
| Author | Santos, Mariana Silva dos | |
| Author | Doro, Daniel | |
| Author | Fernandes, Ana Paula | |
| Author | Freitas, Eloísa de | |
| Author | Michalick, Marilene Susan Marques | |
| Author | Grimaldi, Gabriel | |
| Access date | 2014-03-11T13:23:20Z | |
| Available date | 2014-03-11T13:23:20Z | |
| Document date | 2012 | |
| Citation | COSTA, Míriam Maria et al. Improved canine and human visceral leishmaniasis immunodiagnosis using combinations of synthetic peptides in enzyme-linked immunosorbent assay. Plos Neglected Tropical Diseases, v. 6, p. 1-9, 2012. | pt_BR |
| ISSN | 1935-2727 | |
| URI | https://www.arca.fiocruz.br/handle/icict/7392 | |
| Language | eng | pt_BR |
| Publisher | Public library of Science | pt_BR |
| Rights | open access | |
| Title | Improved canine and human visceral leishmaniasis immunodiagnosis using combinations of synthetic peptides in enzyme-linked immunosorbent assay | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.pntd.0001622 | |
| Abstract | Background: Zoonotic visceral leishmaniasis (VL) is a severe infectious disease caused by protozoan parasites of the genus Leishmania and the domestic dogs are the main urban parasite reservoir hosts. In Brazil, indirect fluorescence antibody tests (IFAT) and indirect enzyme linked immunosorbent assay (ELISA) using promastigote extracts are widely used in epidemiological surveys. However, their sensitivity and specificity have often been compromised by the use of complex mixtures of antigens, which reduces their accuracy allowing the maintenance of infected animals that favors transmission to humans. In this context, the use of combinations of defined peptides appears favorable. Therefore, they were tested by combinations of five peptides derived from the previously described Leishmania diagnostic antigens A2, NH, LACK and K39.
Methodology/Principal Findings : Combinations of peptides derived A2, NH, LACK and K39 antigens were used in ELISA with sera from 44 human patients and 106 dogs. Improved sensitivities and specificities, close to 100%, were obtained for both sera of patients and dogs. Moreover, high sensitivity and specificity were observed even for canine sera presenting low IFAT anti-Leishmania antibody titers or from asymptomatic animals.
Conclusions/Significance : The use of combinations of B cell predicted synthetic peptides derived from antigens A2, NH, LACK and K39 may provide an alternative for improved sensitivities and specificities for immunodiagnostic assays of VL. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil / University of Massachusetts Medical School. Division of Infectious Diseases and Immunology. Worcester, Ma, USA. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Antibodies | pt_BR |
| Subject | Diagnostic medicine | pt_BR |
| Subject | Dogs | pt_BR |
| Subject | Enzyme-linked immunoassays | pt_BR |
| Subject | Kala-azar | pt_BR |
| Subject | Leishmaniasis | pt_BR |
| Subject | Parasitic diseases | pt_BR |
