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https://www.arca.fiocruz.br/handle/icict/7399
IN VITRO INITIAL IMMUNE RESPONSE AGAINST LEISHMANIA AMAZONENSIS INFECTION IS CHARACTERIZED BY AN INCREASED PRODUCTION OF IL-10 AND IL-13
Imunidade Humoral/imunologia
Interleucina-10/biossíntese
Interleucina-13/biossíntese
Leishmania mexicana/imunologia
Leucócitos Mononucleares/parasitologia
Citocinas/biossíntese
DNA de Protozoário/análise
Ensaio de Imunoadsorção Enzimática
Humanos
Interleucina-10/imunologia
Interleucina-13/imunologia
Leishmania mexicana/fisiologia
Leucócitos Mononucleares/imunologia
RNA de Protozoário/análise
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Author
Affilliation
Universidade Federal do Ceará. Medical School. Fortaleza, CE, Brasil
Universidade Federal do Ceará. Medical School. Fortaleza, CE, Brasil
Universidade Federal do Ceará. Medical School. Fortaleza, CE, Brasil
Universidade Federal do Ceará. Medical School. Fortaleza, CE, Brasil
Universidade de São Paulo. Medical School of Ribeirão Preto. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal do Ceará. Medical School. Fortaleza, CE, Brasil
Universidade Federal do Ceará. Medical School. Fortaleza, CE, Brasil
Universidade Federal do Ceará. Medical School. Fortaleza, CE, Brasil
Universidade Federal do Ceará. Medical School. Fortaleza, CE, Brasil
Universidade de São Paulo. Medical School of Ribeirão Preto. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal do Ceará. Medical School. Fortaleza, CE, Brasil
Abstract
The initial encounter of Leishmania with its host's immune system is important in the outcome of infection. Previous studies have shown that PBMCs from healthy volunteers (HV) exposed to Leishmania differ in IFN-γ production. We have expanded such observations evaluating the profile and kinetics of cytokines (IFN-γ, IL-12p70, IL-10, IL-13), chemokines (CCL5, CCL3, CCL4, CXCL10), and chemokine receptors (CCR1,CCR5, CXCR3, CCR4) in vitro L. amazonensis-stimulated of HV's PBMCs. HVs were divided in groups of high (HR) or low (LR) IFN-γ responders. In both groups, HR and LR, after L. amazonensis infection there was a predominance of IL-10 and IL-13 over IFN-γ production, while IL-12 was produced in similar amount. Regarding chemokines, a more striking difference was observed for CCL3 expression that was lower at 12 hours and 48 hours post infection in LR than in HR. Interestingly, a downregulation of CCR5 and a greater expression of CCR4 were found in low IFN-γ responders. These data suggest that early after L. amazonensis infection there is a cytokine milieu dominated by IL-13 and IL-10, and despite of this environment, IFN-γ is produced, supporting the complexity of the response. It is noteworthy that the pattern of immune response is mounted in first hours after Leishmania stimulation, with the definition of the differentiation of Th1 versus Th2 cells. It remains to be determined if such an in vitro difference has an in vivo counterpart in terms of susceptibility to infection.
DeCS
Interações Hospedeiro-Parasita/imunologiaImunidade Humoral/imunologia
Interleucina-10/biossíntese
Interleucina-13/biossíntese
Leishmania mexicana/imunologia
Leucócitos Mononucleares/parasitologia
Citocinas/biossíntese
DNA de Protozoário/análise
Ensaio de Imunoadsorção Enzimática
Humanos
Interleucina-10/imunologia
Interleucina-13/imunologia
Leishmania mexicana/fisiologia
Leucócitos Mononucleares/imunologia
RNA de Protozoário/análise
Reação em Cadeia da Polimerase Via Transcriptase Reversa
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