Author | Balestieri, Filomena Maria Perrella | |
Author | Queiroz, Allan R. Pires | |
Author | Scavone, Cristoforo | |
Author | Costa, Vlaudia M. Assis | |
Author | Barral Netto, Manoel | |
Author | Abrahamsohn, Ises de Almeida | |
Access date | 2014-03-21T19:19:07Z | |
Available date | 2014-03-21T19:19:07Z | |
Document date | 2002 | |
Citation | BALESTIERI, F. et al. Leishmania (L.) amazonensis-induced inhibition of nitric oxide synthesis in host macrophages. Microbes and Infection, v. 4, p. 23-29, 2002. | pt_BR |
ISSN | 1286-4579 | |
xmlui.metadata.dc.identifier.other | S 1 2 8 6 - 4 5 7 9 ( 0 1 ) 0 1 5 0 5 - 2 | |
URI | https://www.arca.fiocruz.br/handle/icict/7437 | |
Language | eng | pt_BR |
Publisher | Elsevier SAS | pt_BR |
Rights | open access | pt_BR |
Title | Leishmania (L.) amazonensis-induced inhibition of nitric oxide synthesis in host macrophages | pt_BR |
Type | Article | pt_BR |
Abstract | Inhibition of lipopolysaccharide (LPS)-induced nitric oxide (NO) production was demonstrated in J774-G8 macrophages infected with Leishmania (L.) amazonensis promastigotes. The downmodulation of NO production observed in infected and LPS-stimulated J774-G8 cells correlated with a reduction in inducible nitric oxide synthase (iNOS) activity. Reduction in iNOS activity was not paralleled by decreased iNOS mRNA expression, suggesting that the parasite affects post-transcriptional events of NO synthesis. Supplementation with L-arginine or tetrahydrobiopterin did not increase NO production, suggesting that inhibition is not due to an insufficiency of substrate or co-factor. Treatment with anti-IL-10, anti-IL-4 or anti-TGF-beta neutralizing antibodies also failed to increase NO production, indicating that these cytokines are not involved in the observed parasite-induced inhibition of NO synthesis. However, treatment of the cultures with IFN-gamma resulted in a marked increase in NO production by infected LPS-stimulated cells. These results show that although L.(L.) amazonensis infection inhibits iNOS activity and NO production by J774-G8 cells, activation by IFN-gamma is capable of overriding the suppression of NO synthesis. | pt_BR |
Affilliation | Universidade Federal da Paraíba. Departamento de Fisiologia e Patologia. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade de São Paulo. ICB. Departamento de Farmacologia. Cidade Universitária. São Paulo, SP, Brasil | pt_BR |
Affilliation | Universidade de São Paulo. ICB. Departamento de Imunologia. Cidade Universitária. São Paulo, SP, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade de São Paulo. ICB. Departamento de Imunologia. Cidade Universitária. São Paulo, SP, Brasil | pt_BR |
Subject | Leishmania (L.) amazonensis | pt_BR |
Subject | Macrophage | pt_BR |
Subject | Nitric oxide | pt_BR |
Subject | INOS activity | pt_BR |
Subject | INOS mRNA | pt_BR |
Subject | IFN-γ | pt_BR |
Subject | Animais | pt_BR |
Subject | Células Cultivadas | pt_BR |
DeCS | Leishmania/patogenicidade | pt_BR |
DeCS | Leishmaniose/parasitologia | pt_BR |
DeCS | Macrófagos/parasitologia | pt_BR |
DeCS | Óxido Nítrico/biossíntese | pt_BR |
DeCS | Lipopolissacarídeos/farmacologia | pt_BR |
DeCS | Macrófagos/efeitos de drogas | pt_BR |
DeCS | Macrófagos/metabolismo | pt_BR |
DeCS | Camundongos | pt_BR |
DeCS | Óxido Nítrico Sintase/genética | pt_BR |
DeCS | Óxido Nítrico Sintase/metabolismo | pt_BR |
DeCS | Óxido Nítrico Sintase Tipo II | pt_BR |
DeCS | RNA Mensageiro/genética | pt_BR |
DeCS | RNA Mensageiro/metabolismo | pt_BR |