| Author | Grangeiro, Thalles Barbosa | |
| Author | Schriefer, Albert | |
| Author | Calvete, Juan J | |
| Author | Raida, Manfred | |
| Author | Urbanke, Claus | |
| Author | Barral Netto, Manoel | |
| Author | Cavada, Benildo Sousa | |
| Access date | 2014-03-26T14:05:44Z | |
| Available date | 2014-03-26T14:05:44Z | |
| Document date | 1997 | |
| Citation | GRANGEIRO, T. B. et al. Molecular cloning and characterization of ConBr, the lectin of Canavalia brasiliensis seeds. Eur.opean Journal of Biochemistry, v. 248, n. 1, p. 43-48, 1997. | pt_BR |
| ISSN | 0014-2956 | |
| URI | https://www.arca.fiocruz.br/handle/icict/7449 | |
| Language | eng | pt_BR |
| Publisher | Springer Verlag | pt_BR |
| Rights | open access | pt_BR |
| Title | Molecular cloning and characterization of ConBr, the lectin of Canavalia brasiliensis seeds | pt_BR |
| Type | Article | pt_BR |
| Abstract | ConBr, a lectin isolated from Canavalia brusiliensis seeds, shares with other legume plant lectins
from the genus Canavaliu (Diocleinae subtribe) primary carbohydrate recognition specificity for D-mannose
and D-glucose. However, ConBr exerts different biological effects than concanavalin A, the lectin
of Canavalia ensiformis seeds, regarding induction of rat paw edema, peritoneal macrophage spreading
in mouse, and in vitro human lymphocyte stimulation. The primary structure of ConBr was established
by cDNA cloning, amino acid sequencing, and mass spectrometry. The 237-amino-acid sequence of
ConBr displays Ser/Thr heterogeneity at position 96, indicating the existence of two isoforms. The mature
Canavalia brasiliensis lectin monomer consists of a mixture of predominantly full-length polypeptide
(a-chain) and a small proportion of fragments 1-118 (J-chain) and 119-237 (y-chain). Although ConBr
isolectins and concanavalin A differ only in residues at positions 58, 70, and 96, ConBr monomers
associate into dimers and tetramers in a different pH-dependent manner than those of concanavalin A.
The occurrence of glycine at position 58 does not allow formation of the hydrogen bond that in the
concanavalin A tetramer exists between Asp58 of subunit A and Sec62 of subunit C. The consequence is
that the a carbons of the corresponding residues in ConBr are 1.5 A closer that in concanavalin A, and
ConBr adopts a more open quaternary structure than concanavalin A. Our data support the hypothesis
that substitution of amino acids located at the subunit interface of structurally related lectins of the same
protein family can lead to different quaternary conformations that may account for their different biological
activities. | pt_BR |
| Affilliation | Universidade Federal do Ceará. Departamento de Bioquimica e Biologia Molecular. Laboratdrio de Lectinas. Centro de Ciências. Fortaleza, CE, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Institut fur Reproduktionsmedizin. Tierarztliche Hochschule. Hannover, Germany | pt_BR |
| Affilliation | Niedersachsisches Institut fur Peptid-Forschung GmbH. Hannover, Germany | pt_BR |
| Affilliation | Biophysikalische MeRgerateabteilung. Medizinische Hochschule. Hannover, Germany | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal do Ceará. Departamento de Bioquimica e Biologia Molecular. Laboratdrio de Lectinas. Centro de Ciências. Fortaleza, CE, Brasil | pt_BR |
| Subject | Legume lectin | pt_BR |
| Subject | Canavalia brasiliensis | pt_BR |
| Subject | Nucleotide sequence | pt_BR |
| Subject | Amino acid sequence ; mass spectrometry | pt_BR |
| DeCS | Fabaceae/genética | pt_BR |
| DeCS | Lectinas/genética | pt_BR |
| DeCS | Plantas Medicinais | pt_BR |
| DeCS | Sequência de Aminoácidos | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Sequência de Bases | pt_BR |
| DeCS | Sítios de Ligação | pt_BR |
| DeCS | Clonagem Molecular | pt_BR |
| DeCS | Primers do DNA/genética | pt_BR |
| DeCS | Edema/etiologia | pt_BR |
| DeCS | Genes de Plantas | pt_BR |
| DeCS | Ligações de Hidrogênio | pt_BR |
| DeCS | Humanos | pt_BR |
| DeCS | Concentração de Íons de Hidrogênio | pt_BR |
| DeCS | Modelos Moleculares | pt_BR |
| DeCS | Reação em Cadeia da Polimerase | pt_BR |
