Author | Gomes, Luciana Inácia | |
Author | Gonzaga, Felipe M. | |
Author | Teixeira, Eliane de Morais | |
Author | Rodrigues, Bruna Soares de Souza Lima | |
Author | Freire, Verônica V. | |
Author | Rabello, Ana Lúcia Teles | |
Access date | 2014-03-26T18:48:08Z | |
Available date | 2014-03-26T18:48:08Z | |
Document date | 2012 | |
Citation | GOMES, Luciana Inácia et al. Validation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity. Experimental Parasitology. 2012, vol.131, pp. 175-179 | pt_BR |
ISSN | 0014-4894 | |
URI | https://www.arca.fiocruz.br/handle/icict/7458 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Title | Validation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.exppara.2012.03.021 | |
Abstract | In vitro assays play an important role in the discovery and development of new antileishmanial drugs. The classic macrophage-amastigote models using murine peritoneal macrophages or human-monocyte derived macrophages as host cells are useful for drug screening. A major limitation of these models is the dependence on microscopic counting, a time-consuming and subjective method of analysis. The present study describes a detailed protocol for applying quantitative real-time PCR (qPCR) as an accurate and sensitive tool to assess parasite load in an amastigote-macrophage model. This assay can be performed in a standardized medium-to-high throughput procedure, replacing traditional readout of number of amastigote per macrophages by DNA load measurement. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Clinical Research. Belo Horizonte, Minas Gerais, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Clinical Research. Belo Horizonte, Minas Gerais, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Clinical Research. Belo Horizonte, Minas Gerais, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Clinical Research. Belo Horizonte, Minas Gerais, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Clinical Research. Belo Horizonte, Minas Gerais, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Clinical Research. Belo Horizonte, Minas Gerais, Brazil | pt_BR |
Subject | antileishmanial drugs | pt_BR |
Subject | Leishmania | pt_BR |
Subject | In vitro assays | pt_BR |
Subject | Quantitative real-time PCR | pt_BR |