| Author | Shoemaker, Charles B | |
| Author | Ramachandran, Hema | |
| Author | Landa, Abraham | |
| Author | Reis, Mitermayer Galvão dos | |
| Author | Stein, Lincoln D | |
| Access date | 2014-05-06T11:53:58Z | |
| Available date | 2014-05-06T11:53:58Z | |
| Document date | 1992 | |
| Citation | SHOEMAKER, C. et al. Alternative splicing of the Schistosoma mansoni gene encoding a homologue of epidermal growth factor receptor. Molecular and Biochemical Parasitology, v. 53, n. 1-2, p. 17-32, 1992. | pt_BR |
| ISSN | 0166-6851 | |
| URI | https://www.arca.fiocruz.br/handle/icict/7581 | |
| Language | eng | pt_BR |
| Publisher | Elsevier Science Publishers B.V | pt_BR |
| Rights | open access | pt_BR |
| Title | Alternative splicing of the Schistosoma mansoni gene encoding a homologue of epidermal growth factor receptor | pt_BR |
| Type | Article | pt_BR |
| Abstract | The complete coding DNA for a Schistosoma mansoni homologue of the epidermal growth factor receptor (SER) was
characterized from cDNA clones obtained by homology to the tyrosine kinase domain of erbB. The DNA sequence predicts a
200-kDa translation product that contains a secretory leader, a cysteine-rich extracellular domain, a hydrophobic
transmembrane sequence, and an intracellular tyrosine kinase domain. The SER transcript is present in cercariae and adult
schistosomes.
In addition to SER transcripts, schistosomes produce at least 3 variant transcripts encoding truncated SER products that
include the secretory leader and a small portion of the extracellular domain followed by short sequences of unrelated, Cterminal
amino acids. Based on these sequences, 2 of the variant mRNAs (class 2 and 5) appear to encode soluble, secreted
proteins while one (class 4) encodes an SER variant protein with a hydrophobic C-terminus that may serve as a membrane
anchor. Class 2 SER variant transcripts are present at levels comparable to SER transcripts in adult worms but are not
detected in cercariae. Class 4 and 5 SER variant transcripts are also found within adult worms but at lower levels. Genomic
cloning and characterization demonstrate that the variant SER transcripts arise through alternative splicing of the SER gene | pt_BR |
| Affilliation | Department of Tropical Public Health. Harvard School of Public Health. Boston, MA, USA | pt_BR |
| Affilliation | Department of Tropical Public Health. Harvard School of Public Health. Boston, MA, USA | pt_BR |
| Affilliation | Department of Immunology. Inst. de Investigaciones Biomédicas. UNAM. México, D.F, Mexico | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Deptartment of Pathology. Brigham and Women's Hospital. Harvard Medical School. Boston, MA, USA | pt_BR |
| Subject | Schistosome | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| Subject | EGF | pt_BR |
| Subject | EGF receptor | pt_BR |
| Subject | Alternative splicing | pt_BR |
| Subject | Membrane protein | pt_BR |
| DeCS | Processamento de RNA | pt_BR |
| DeCS | Receptor do Fator de Crescimento Epidérmico/genética | pt_BR |
| DeCS | Schistosoma mansoni/genética | pt_BR |
| DeCS | Sequência de Aminoácidos | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Sequência de Bases | pt_BR |
| DeCS | Clonagem Molecular | pt_BR |
| DeCS | DNA/genética | pt_BR |
| DeCS | Proteínas de Helminto/genética | pt_BR |
| DeCS | Dados de Sequência Molecular | pt_BR |
| DeCS | RNA Mensageiro/genética | pt_BR |
| DeCS | Homologia de Sequência do Ácido Nucleico | pt_BR |
| DeCS | Transcrição Genética | pt_BR |
