| Author | Assis, Girley Francisco Machado de | |
| Author | Silva, Alexandre Rotondo da | |
| Author | Do Bem, V. A. L. | |
| Author | Bahia, Maria Terezinha | |
| Author | Martins Filho, Olindo Assis | |
| Author | Dias, João Carlos Pinto | |
| Author | Albajar-Viñas, Pedro | |
| Author | Torres, Rosália Morais | |
| Author | Lana, Marta de | |
| Access date | 2014-05-28T12:18:12Z | |
| Available date | 2014-05-28T12:18:12Z | |
| Document date | 2012 | |
| Citation | MACHADO DE ASSIS, Girley Francisco et al. Posttherapeutic cure criteria in Chagas' disease: conventional serology followed by supplementary serological, parasitological, and molecular tests. Clinical and Vaccine Immunology. 2012, vol.19, n.8, 1283-1291 | pt_BR |
| ISSN | 1556-6811 | |
| URI | https://www.arca.fiocruz.br/handle/icict/7655 | |
| Language | eng | pt_BR |
| Publisher | American Society for Microbiology | pt_BR |
| Rights | open access | pt_BR |
| Title | Posttherapeutic cure criteria in Chagas' disease: conventional serology followed by supplementary serological, parasitological, and molecular tests | pt_BR |
| Type | Article | pt_BR |
| Abstract | We performed a critical study of conventional serology, followed by supplementary serological, parasitological, and molecular tests, to assess the response to etiologic treatment of Chagas' disease. A group of 94 Chagas' disease patients treated with benznidazole at least 10 years earlier were evaluated from the laboratory and clinical points of view. When conventional serology (enzyme-linked immunosorbent assay [ELISA], indirect immunofluorescence [IIF], and indirect hemagglutination [IHA]) and classic criteria (consistent results with any two of the three tests) or more rigorous criteria (consistent results from the three tests) were used, 10.6% and 8.5% of patients were considered treated and cured (TC) by classic and rigorous criteria, respectively. Patients were then evaluated using supplementary (recombinant ELISA and Trypanosoma cruzi excreted-secreted antigen blotting [TESA-blot]), parasitological (hemoculture), and molecular (PCR) tests. The results of recombinant ELISA were similar to those with the rigorous criterion (three consistent test results). The TESA-blot group showed a higher percentage (21.3%) of negative results than the groups defined by either cure criterion. Hemoculture and PCR gave negative results for all treated and cured (TC) patients, regardless of the criterion used. Recombinant ELISA and TESA-blot tests showed negative results for 70% and 87.5% of the patients categorized as TC by the classic and three-test criteria, respectively. For patients with discordant conventional serology, the supplementary serological and molecular tests were the decisive factor in determining therapeutic failure. Clinical evaluation showed that 62.5% of TC patients presented with the indeterminate form of the disease. Additionally, treated patients with negative TESA-blot results should be reevaluated later with all methodologies used here to verify whether TESA-blot is a reliable way to determine early parasitological cure of Chagas' disease. | pt_BR |
| Affilliation | Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Organização Mundial de Saúde. Departamento de Controle de Doenças Tropicais Negligenciadas. Geneva, Switzerland | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, Brazil/Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Análises Clínicas. Ouro Preto, MG, Brazil | pt_BR |
| Subject | Chagas' disease | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
