| Author | Assis, Tália Santana Machado de | |
| Author | Rabello, Ana Lúcia Teles | |
| Access date | 2014-05-28T14:06:57Z | |
| Available date | 2014-05-28T14:06:57Z | |
| Document date | 2012 | |
| Citation | ASSIS, Talia Santana Machado de; RABELLO, Ana Lucia Teles; WERNECK, Guilherme Loureiro. Latent class analysis of diagnostic tests for visceral leishmaniasis in Brazil. Tropical Medicine and International Health. 2012, vol.17, pp. 1202-1207 | pt_BR |
| ISSN | 1360-2276 | |
| URI | https://www.arca.fiocruz.br/handle/icict/7657 | |
| Language | eng | pt_BR |
| Publisher | Belgian Society of Tropical Medicine | pt_BR |
| Rights | open access | pt_BR |
| Title | Latent class analysis of diagnostic tests for visceral leishmaniasis in Brazil | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1111/j.1365-3156.2012.03064.x | |
| Abstract | Objective To estimate the sensitivities and specificities of different diagnostic tests for visceral leishmaniasis (VL) using latent class analysis (LCA).
Methods This study was performed using data from a prospective study conducted in four Brazilian states from May 2004 to May 2007. Five diagnostic tests for VL were evaluated in 285 VL cases and 119 non-cases: microscopy, indirect fluorescence antibody test (IFAT), enzyme-linked immunosorbent assay using recombinant K39 antigen (rK39-ELISA), direct agglutination test (DAT) and the rK39 rapid test.
Results Microscopy showed sensitivity of 77.0% (CI: 71.5–81.5) and specificity of 99.0% (CI: 94.0–99.7). The IFAT and the DAT showed similar sensitivities, 88.3% (CI: 84.0–92.0) and 88.5% (CI: 84.1–92.0), respectively, but the DAT had a higher specificity (95.4%, CI: 89.2–98.1) than did the IFAT (83.0%, CI: 75.0–88.2). The rK39-ELISA and the rK39 rapid test showed sensitivities of 99.0% (CI: 96.3–99.6) and 94.0% (CI: 90.1–96.3), and specificities of 82.5% (CI: 75.0–88.3) and 100% (CI: 97.0–100.0%), respectively.
Conclusions Considering the lack of an adequate reference standard, LCA proved to be a useful tool in validating diagnostic methods for VL. The DAT and the rK39 rapid test showed better performance. Thus, clinically suspected cases of VL in a Brazilian endemic area could be treated based on the positivity of one of these tests. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Instituto de Medicina Social. Departamento de Epidemiologia. Rio de Janeiro, Brazil | pt_BR |
| Subject | visceral leishmaniasis | pt_BR |
| Subject | diagnosis | pt_BR |
| Subject | latent class analysis | pt_BR |
