| Author | Penha, Euler Moraes | |
| Author | Meira, Cássio Santana | |
| Author | Guimarães, Elisalva Teixeira | |
| Author | Mendonça, Marcus Vinícius Pinheiro | |
| Author | Gravely, Faye Alice | |
| Author | Pinheiro, Cláudia Maria Bahia | |
| Author | Pinheiro, Taiana Maria Bahia | |
| Author | Melo, Stella Maria Barrouin | |
| Author | Santos, Ricardo Ribeiro dos | |
| Author | Soares, Milena Botelho Pereira | |
| Access date | 2014-06-03T19:00:40Z | |
| Available date | 2014-06-03T19:00:40Z | |
| Document date | 2014 | |
| Citation | PENHA, E. M. et al. Use of autologous mesenchymal stem cells derived from bone marrow for the treatment of naturally injured spinal cord in dogs. Stem Cells International, p.37521, 2014. | pt_BR |
| ISSN | 1687-966X | |
| URI | https://www.arca.fiocruz.br/handle/icict/7764 | |
| Language | eng | pt_BR |
| Publisher | Hindawi Publishing Corporation | pt_BR |
| Rights | open access | pt_BR |
| Title | Use of autologous mesenchymal stem cells derived from bone marrow for the treatment of naturally injured spinal cord in dogs | pt_BR |
| Type | Article | pt_BR |
| DOI | dx.doi.org/10.1155/2014/437521 | |
| Abstract | The use of stem cells in injury repair has been extensively investigated. Here, we examined the therapeutic effects of autologous bone marrow mesenchymal stem cells (MSC) transplantation in four dogs with natural traumatic spinal cord injuries. MSC were cultured in vitro, and proliferation rate and cell viability were evaluated. Cell suspensions were prepared and surgically administered into the spinal cord. The animals were clinically evaluated and examined by nuclear magnetic resonance. Ten days after the surgical procedure and MSC transplantation, we observed a progressive recovery of the panniculus reflex and diminished superficial and deep pain response, although there were still low proprioceptive reflexes in addition to a hyperreflex in the ataxic hind limb movement responses. Each dog demonstrated an improvement in these gains over time. Conscious reflex recovery occurred simultaneously with moderate improvement in intestine and urinary bladder functions in two of the four dogs. By the 18th month of clinical monitoring, we observed a remarkable clinical amelioration accompanied by improved movement, in three of the four dogs. However, no clinical gain was associated with alterations in magnetic resonance imaging. Our results indicate that MSC are potential candidates for the stem cell therapy following spinal cord injury. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital de Medicina Veterinária. Escola de Medicina Veterinária e Zootecnia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade do Estado da Bahia. Departamento de Ciências da Vida. Salvador, BA, Brasil | pt_BR |
| Affilliation | Hospital Espanhol. Salvador, BA, Brasil | pt_BR |
| Affilliation | A Arca Veterinária. Salvador, BA, Brasil | pt_BR |
| Affilliation | Estácio-FIB. Centro Universitário Estácio da Bahia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Estácio-FIB. Centro Universitário Estácio da Bahia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospital de Medicina Veterinária. Escola de Medicina Veterinária e Zootecnia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Centro de Biotecnologia e Terapia Celular. Hospital São Rafael. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Centro de Biotecnologia e Terapia Celular. Hospital São Rafael. Salvador, BA, Brasil | pt_BR |
| DeCS | Células-tronco | pt_BR |
| DeCS | Medula óssea | pt_BR |
| DeCS | Medula espinhal | pt_BR |
| DeCS | Transplante | pt_BR |
| DeCS | Cirurgia | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Cães | pt_BR |
| DeCS | Ressonância magnética | pt_BR |
