| Author | Protasio, Anna V. | |
| Author | Tsai, Isheng J. | |
| Author | Babbage, Anne | |
| Author | Nichol, Sarah | |
| Author | Hunt, Martin | |
| Author | Aslett, Martin A. | |
| Author | Silva, Nishadi de | |
| Author | Velarde, Giles S. | |
| Author | Clark, Richard C. | |
| Author | Davidson, Claire | |
| Author | Dillon, Gary P. | |
| Author | Holroyd, Nancy E. | |
| Author | Lloyd, Christine | |
| Author | McQuillan, Jacquelline | |
| Author | Otto, Thomas D. | |
| Author | Quail, Michael A. | |
| Author | Berriman, Matthew | |
| Author | Anderson, Tim J. C. | |
| Author | LoVerde, Phillip Thomas | |
| Author | Oliveira, Guilherme Corrêa de | |
| Author | Zerlotini, Adhemar | |
| Author | Manuel, Sophia J. Parker | |
| Author | Wilson, R. Alan | |
| Author | Dunne, David W. | |
| Access date | 2014-06-24T18:14:12Z | |
| Available date | 2014-06-24T18:14:12Z | |
| Document date | 2012 | |
| Citation | PROTASIO, Anna V. et al . A systematically improved high quality genome and transcriptome of the human blood fluke Schistosoma mansoni. Plos Neglected Tropical Diseases. 2012, vol.6, pp. e1455. | pt_BR |
| ISSN | 1935-2735 | |
| URI | https://www.arca.fiocruz.br/handle/icict/7836 | |
| Sponsorship | Wellcome Trust (grant WT 085775/Z/08/Z to M.B.), National Institutes of Health-Fogarty (TW007012), CNPq (573839/2008-
5), and FAPEMIG (1181/08 and PPM-00439-10 to G.O.). Some schistosome materials were provided by the National Institute of Allergy and Infectious Diseases (NIAID) Schistosomiasis Resource Center (NIAID contract no. HHSN272201000009I). | pt_BR |
| Language | eng | pt_BR |
| Publisher | Public Library of Science, Public Library of Science | pt_BR |
| Rights | open access | pt_BR |
| Title | A systematically improved high quality genome and transcriptome of the human blood fluke Schistosoma mansoni | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1371/journal.pntd.0001455 | |
| Abstract | Schistosomiasis is one of the most prevalent parasitic diseases, affecting millions of people in developing countries. Amongst the human-infective species, Schistosoma mansoni is also the most commonly used in the laboratory and here we present the systematic improvement of its draft genome. We used Sanger capillary and deep-coverage Illumina sequencing from clonal worms to upgrade the highly fragmented draft 380 Mb genome to one with only 885 scaffolds and more than 81% of the bases organised into chromosomes. We have also used transcriptome sequencing (RNA-seq) from four time points in the parasite's life cycle to refine gene predictions and profile their expression. More than 45% of predicted genes have been extensively modified and the total number has been reduced from 11,807 to 10,852. Using the new version of the genome, we identified trans-splicing events occurring in at least 11% of genes and identified clear cases where it is used to resolve polycistronic transcripts. We have produced a high-resolution map of temporal changes in expression for 9,535 genes, covering an unprecedented dynamic range for this organism. All of these data have been consolidated into a searchable format within the GeneDB (www.genedb.org) and SchistoDB (www.schistodb.net) databases. With further transcriptional profiling and genome sequencing increasingly accessible, the upgraded genome will form a fundamental dataset to underpin further advances in schistosome research. | pt_BR |
| Affilliation | Wellcome Trust Sanger Institute. Wellcome Trust Genome Campus. Hinxton, United Kingdom | pt_BR |
| Affilliation | Wellcome Trust Sanger Institute. Wellcome Trust Genome Campus. Hinxton, United Kingdom | pt_BR |
| Affilliation | Wellcome Trust Sanger Institute. Wellcome Trust Genome Campus. Hinxton, United Kingdom | pt_BR |
| Affilliation | Wellcome Trust Sanger Institute. Wellcome Trust Genome Campus. Hinxton, United Kingdom | pt_BR |
| Affilliation | Wellcome Trust Sanger Institute. Wellcome Trust Genome Campus. Hinxton, United Kingdom | pt_BR |
| Affilliation | Wellcome Trust Sanger Institute. Wellcome Trust Genome Campus. Hinxton, United Kingdom | pt_BR |
| Affilliation | Wellcome Trust Sanger Institute. Wellcome Trust Genome Campus. Hinxton, United Kingdom | pt_BR |
| Affilliation | Wellcome Trust Sanger Institute. Wellcome Trust Genome Campus. Hinxton, United Kingdom | pt_BR |
| Affilliation | Wellcome Trust Sanger Institute. Wellcome Trust Genome Campus. Hinxton, United Kingdom | pt_BR |
| Affilliation | Wellcome Trust Sanger Institute. Wellcome Trust Genome Campus. Hinxton, United Kingdom | pt_BR |
| Affilliation | Wellcome Trust Sanger Institute. Wellcome Trust Genome Campus. Hinxton, United Kingdom | pt_BR |
| Affilliation | Wellcome Trust Sanger Institute. Wellcome Trust Genome Campus. Hinxton, United Kingdom | pt_BR |
| Affilliation | Wellcome Trust Sanger Institute. Wellcome Trust Genome Campus. Hinxton, United Kingdom | pt_BR |
| Affilliation | Wellcome Trust Sanger Institute. Wellcome Trust Genome Campus. Hinxton, United Kingdom | pt_BR |
| Affilliation | Wellcome Trust Sanger Institute. Wellcome Trust Genome Campus. Hinxton, United Kingdom | pt_BR |
| Affilliation | Wellcome Trust Sanger Institute. Wellcome Trust Genome Campus. Hinxton, United Kingdom | pt_BR |
| Affilliation | Wellcome Trust Sanger Institute. Wellcome Trust Genome Campus. Hinxton, United Kingdom | pt_BR |
| Affilliation | Texas Biomedical Research Institute. San Antonio, TX, United States of America | pt_BR |
| Affilliation | University of Texas Health Science Center. Departments of Biochemistry and Pathology. San Antonio, TX, United States of America | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Centro de Excelência em Bioinformatica. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Genomics and Computational Biology Group. Belo Horizonte, Minas Gerais, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. National Institute for Science and Technology in Tropical Diseases. Belo Horizonte, Minas Gerais, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Centro de Excelência em Bioinformatica. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | University of York. Department of Biology. Heslington, York, United Kingdom | pt_BR |
| Affilliation | University of York. Department of Biology. Heslington, York, United Kingdom | pt_BR |
| Affilliation | University of Cambridge. Department of Pathology. Cambridge, United Kingdom | pt_BR |
| Subject | DNA sequences | pt_BR |
| Subject | genes expression | pt_BR |
| Subject | genomics databases | pt_BR |
| Subject | genomics libraries | pt_BR |
| Subject | invertebrates genomics | pt_BR |
| Subject | parasitic life cycles | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| Subject | sequence assembly | pt_BR |
