Author | Silva, Elisângela Fátima | |
Author | Orsi, Mariana | |
Author | Andrade, Angela Leão | |
Author | Domingues, Rosana Zacarias | |
Author | Silva, Breno de Mello | |
Author | Araújo, Helena Rocha Corrêa de | |
Author | Pimenta, Paulo Filemon Paolucci | |
Author | Diamond, Michael S. | |
Author | Rocha, Eliseu Soares de Oliveira | |
Author | Kroon, Erna Geessien | |
Author | Malaquias, Luiz Cosme Cotta | |
Author | Coelho, Luiz Felipe Leomil | |
Access date | 2014-07-14T13:53:42Z | |
Available date | 2014-07-14T13:53:42Z | |
Document date | 2012 | |
Citation | SILVA, Elisângela Fátima et al. A tetravalent dengue nanoparticle stimulates antibody production in mice. Journal of Nanobiotechnology. 2012, vol.10, pp. 13 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/7945 | |
Sponsorship | FAPEMIG and CNPq | pt_BR |
Language | eng | pt_BR |
Publisher | BioMed Central | pt_BR |
Rights | open access | pt_BR |
Title | A tetravalent dengue nanoparticle stimulates antibody production in mice. Journal of Nanobiotechnology | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/1477-3155-10-13 | |
Abstract | Background: Dengue is a major public health problem worldwide, especially in the tropical and subtropical regions of the world. Infection with a single Dengue virus (DENV) serotype causes a mild, self-limiting febrile illness called dengue fever. However, a subset of patients experiencing secondary infection with a different serotype progresses to the severe form of the disease, dengue hemorrhagic fever/dengue shock syndrome. Currently, there are no licensed vaccines or antiviral drugs to prevent or treat dengue infections. Biodegradable nanoparticles coated with proteins represent a promising method for in vivo delivery of vaccines.
Findings: Here, we used a murine model to evaluate the IgG production after administration of inactivated DENV corresponding to all four serotypes adsorbed to bovine serum albumin nanoparticles. This formulation induced a production of anti-DENV IgG antibodies (p < 0.001). However, plaque reduction neutralization assays with the four DENV serotypes revealed that these antibodies have no neutralizing activity in the dilutions tested.
Conclusions: Our results show that while the nanoparticle system induces humoral responses against DENV, further investigation with different DENV antigens will be required to improve immunogenicity, epitope specicity, and functional activity to make this platform a viable option for DENV vaccines. | pt_BR |
Affilliation | Universidade Federal de Alfenas. Instituto de Ciências Biomédicas. Alfenas, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Alfenas. Instituto de Ciências Biomédicas. Alfenas, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Ouro Preto. Departamento de Química. Ouro Preto, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Departamento de Química. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Ouro Preto. Departamento de Ciências Biológicas. Ouro Preto, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Washington University School of Medicine. Departments of Medicine, Molecular Microbiology, Pathology & Immunology. Saint Louis, Missouri, USA. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biomédicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biomédicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Alfenas. Instituto de Ciências Biomédicas. Alfenas, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Alfenas. Instituto de Ciências Biomédicas. Alfenas, MG, Brazil. | pt_BR |
Subject | inactivated Dengue vírus | pt_BR |
Subject | Nanoparticles | pt_BR |
Subject | humoral response | pt_BR |