| Author | Soares, Rodrigo Pedro Pinto | |
| Author | Torrecilhas, Ana Claudia Trocoli | |
| Author | Assis, Rafael Ramiro de | |
| Author | Rocha, Marcele Neves | |
| Author | Castro, Felipe A. Moura e | |
| Author | Freitas, Gustavo F. | |
| Author | Murta, Silvane Maria Fonseca | |
| Author | Santos, Sara Lopes dos | |
| Author | Marques, Alexandre Ferreira | |
| Author | Almeida, Igor Correia de | |
| Author | Romanha, Álvaro José | |
| Access date | 2014-07-21T14:29:31Z | |
| Available date | 2014-07-21T14:29:31Z | |
| Document date | 2012 | |
| Citation | SOARES, Rodrigo Pedro Pinto et al. Intraspecies variation in Trypanosoma cruzi GPI-mucins: biological activities and differential expression of -αgalactosyl residues. The American Society of Tropical Medicine and Hygiene. 2012, vol.87, pp. 87-96. | pt_BR |
| ISSN | 0002-9637 | |
| URI | https://www.arca.fiocruz.br/handle/icict/7999 | |
| Language | eng | pt_BR |
| Publisher | American Society of Tropical Medicine and Hygiene | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Intraspecies variation in Trypanosoma cruzi GPI-mucins: biological activities and differential expression of -αgalactosyl residues | pt_BR |
| Type | Article | |
| DOI | 10.4269/ajtmh.2012.12-0015 | |
| Abstract | The glycosylphosphatidylinositol (GPI)-anchored mucins of Trypanosoma cruzi trypomastigotes play an important immunomodulatory role during the course of Chagas disease. Here, some biological activities of tGPI-mucins from four T. cruzi isolates, including benznidazole-susceptible (BZS-Y), benznidazole-resistant (BZR-Y), CL, and Colombiana, were evaluated. GPI-mucins were able to differentially trigger the production of interleukin-12 and nitric oxide in BALB/c macrophages and modulate LLC-MK2 cell invasion. The significance of these variations was assessed after analysis of the terminal α-galactosyl residues. Enzymatic treatment with α-galactosidase indicated a differential expression of O-linked α-galactosyl residues among the strains, with higher expression of this sugar in BZS-Y and BZR-Y T. cruzi populations followed by Colombiana and CL. Unweighted pair group method analysis of the carbohydrate anchor profile and biological parameters allowed the clustering of two groups. One group includes Y and CL strains (T. cruzi II and VI), and the other group is represented by Colombiana strain (T. cruzi I) | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Departamento de Ciências Biológicas. Diadema, SP, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | [s.n.] | pt_BR |
| Affilliation | [s.n.] | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | [s.n.] | pt_BR |
| Affilliation | University of Texas. Department of Biological Sciences. The Border Biomedical Research Center. El Paso, TA, United States of America. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Departamento de Ciências Biológicas. Diadema, SP, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | glycosylphosphatidylinositol | pt_BR |
