| Author | Miranda, Aline Cristina Andrade Mota | |
| Author | Araújo, Sergio Pereira | |
| Author | Dias, Juarez Pereira | |
| Author | Colin, Denise Duizit | |
| Author | Kashima, Simone G | |
| Author | Covas, Dimas Tadeu | |
| Author | Tavares Neto, José | |
| Author | Castro Filho, Bernardo Galvão | |
| Author | Alcantara, Luiz Carlos Júnior | |
| Access date | 2014-07-29T19:02:02Z | |
| Available date | 2014-07-29T19:02:02Z | |
| Document date | 2008 | |
| Citation | MOTA-MIRANDA, A. C. et al. HTLV-1 infection in blood donors from the Western Brazilian Amazon region: seroprevalence and molecular study of viral isolates. Journal of Medical Virology, v. 80, n.11, p. 1966-1971, 2008. | pt_BR |
| ISSN | 1096-9071 | |
| URI | https://www.arca.fiocruz.br/handle/icict/8096 | |
| Language | eng | pt_BR |
| Publisher | Wiley-Liss, INC | pt_BR |
| Rights | restricted access | pt_BR |
| Title | HTLV-1 infection in blood donors from the Western Brazilian Amazon region: seroprevalence and molecular study of viral isolates. | pt_BR |
| Type | Article | |
| Abstract | To determine the seroprevalence of HTLV-1 in Brazil, and to review the virus molecular epidemiology in this Amazon population (Rio Branco-Acre), 219 blood donors were screened for HTLV-1. Only one case of infection (0.46% seroprevalence) was detected during July 2004 screening at the Acre Hospital Foundation (FUNDACRE). Neighbor-joining and Maximum Likelihood phylogenetic analyses of two (n = 2) complete LTR region sequences were performed with the PAUP* software. Since the HTLV-1 envelope surface (gp46) and transmembrane (gp21) glycoproteins are important for virus fitness, three envelope glycoproteins sequences (n = 3) were analyzed using the Prosite tool to determinate potential protein sites. Phylogenetic analysis demonstrated that the new isolate described in this study, and the unpublished LTR strain described in a previous report belong to the Transcontinental subgroup of the Cosmopolitan subtype, inside the Latin American cluster. A similar result was obtained when submitting, to the Automated Genotyping System, three LTR partial sequences from a previous study of the seroprevalence of HTLV-1 in the same Amazon population. In all analyzed env sequences, the potential protein site was found: two PKC phosphorylation sites at amino acid (aa) positions 310-312 and 342-344, one CK2 phosphorylation site at 194-197aa, three N-glycosylation sites at 222-225aa, 244-247aa and 272-275aa, and a single N-myristylation site at 327-338aa. In conclusion, potential protein sites described in HTLV-1 gp46 and gp21 confirm the presence of conserved sites in the HTLV-1 envelope proteins, likewise phylogenetic analysis suggests a possible recent introduction of the virus into North Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Hospital Estadual do Acre. Rio Branco, Acre, Brasil | pt_BR |
| Affilliation | Hemocentro de Ribeirão Preto. São Paulo, SP, Brasil | pt_BR |
| Affilliation | Hemocentro de Ribeirão Preto. São Paulo, SP, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Fundação Bahiana para o Desenvolvimento das Ciências. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Fundação Bahiana para o Desenvolvimento das Ciências. Salvador, BA, Brasil | pt_BR |
| Subject | LTR region | pt_BR |
| Subject | Glycoproteins | pt_BR |
| Subject | Phylogenetic analysis | pt_BR |
| Subject | Protein sites | pt_BR |
| DeCS | Infecções por HTLV-I/epidemiologia | pt_BR |
| DeCS | Vírus 1 Linfotrópico T Humano/classificação | pt_BR |
| DeCS | Vírus 1 Linfotrópico T Humano/isolamento & purificação | pt_BR |
| DeCS | Doadores de Sangue | pt_BR |
| DeCS | Brasil/epidemiologia | pt_BR |
| DeCS | Análise por Conglomerados | pt_BR |
| DeCS | Sequência Conservada | pt_BR |
| DeCS | Produtos do Gene env/genética | pt_BR |
| DeCS | Genótipo | pt_BR |
| DeCS | Vírus 1 Linfotrópico T Humano/genética | pt_BR |
| DeCS | Humanos | pt_BR |
| DeCS | Filogenia | pt_BR |
| DeCS | RNA Viral/genética | pt_BR |
| DeCS | Análise de Sequência de DNA | pt_BR |
| DeCS | Homologia de Sequência | pt_BR |
| DeCS | Estudos Soroepidemiológicos | pt_BR |
