| Author | Grassi, Maria Fernanda Rios | |
| Author | Correa, Ana Beatriz Guimarães | |
| Author | Mascarenhas, Rita Elizabeth Moreira | |
| Author | Castro Filho, Bernardo Galvão | |
| Author | Méniel, Blandine Séon | |
| Author | Schmidt, Fanny | |
| Author | Franck, Xavier | |
| Author | Hocquemiller, Reynald | |
| Author | Figadère, Bruno | |
| Author | Fournet, Alain | |
| Access date | 2014-07-31T14:05:23Z | |
| Available date | 2014-07-31T14:05:23Z | |
| Document date | 2007 | |
| Citation | GRASSI, M. F. R. et al. Quinoline compounds decrease in vitro spontaneous proliferation of peripheral blood mononuclear cells (PBMC) from human T-cell lymphotropic virus (HTLV) type-1-infected patients. Biomedicine & Pharmacotherapr, v. 62, n. 7, p. 430-435, 2008. | pt_BR |
| ISSN | 0753-3322 | |
| URI | https://www.arca.fiocruz.br/handle/icict/8106 | |
| Language | eng | pt_BR |
| Publisher | Elsevier Masson SAS | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Quinoline compounds decrease in vitro spontaneous proliferation of peripheral blood mononuclear cells (PBMC) from human T-cell lymphotropic virus (HTLV) type-1-infected patients. | pt_BR |
| Type | Article | |
| Abstract | In vitro spontaneous proliferation is the immunological hallmark of peripheral blood mononuclear cells (PBMC) from HTLV-1-infected individuals.
Quinoline compounds down regulate in vitro cell proliferation of HTLV-1 transformed cell lines. In the present study we assessed the
capacity of quinolines to inhibit spontaneous cell proliferation of PBMC from HTLV-1-infected individuals. Twenty-two quinolines were evaluated.
Toxicity was first assessed on PBMC from healthy donors by using both the Trypan blue technique and Tetrazolium Salt (XTT) method
and then the antiproliferative effect was measured by a classic lymphoproliferative assay on PBMC from three HTLV-1-infected individuals, in
the presence of decreasing concentrations of quinolines (from 100 mM to 0.8 mM), after 5 days of culture.
We found that 14 out of 22 compounds were non-toxic to PBMC from uninfected individuals at 100, 50 and 10 mM. Four compounds presented
a capacity to inhibit more than 80% of the spontaneous proliferation: 7 at 25 mM and 10, 20 and 23 at 100 mM. Our results indicate that
some quinolines block spontaneous proliferation of PBMC from HTLV-1-infected individuals. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil | pt_BR |
| Affilliation | Université Paris-Sud, Faculté de Pharmacie. Laboratoire de Pharmacognosie-associé au CNRS. Châtenay-Malabry, France | pt_BR |
| Affilliation | Université Paris-Sud, Faculté de Pharmacie. Laboratoire de Pharmacognosie-associé au CNRS. Châtenay-Malabry, France | pt_BR |
| Affilliation | Université Paris-Sud, Faculté de Pharmacie. Laboratoire de Pharmacognosie-associé au CNRS. Châtenay-Malabry, France | pt_BR |
| Affilliation | Université Paris-Sud, Faculté de Pharmacie. Laboratoire de Pharmacognosie-associé au CNRS. Châtenay-Malabry, France | pt_BR |
| Affilliation | Université Paris-Sud, Faculté de Pharmacie. Laboratoire de Pharmacognosie-associé au CNRS. Châtenay-Malabry, France | pt_BR |
| Affilliation | Faculté de Pharmacie. Laboratoire de Pharmacognosie. Châtenay-Malabry, France | pt_BR |
| Subject | HTLV-1 | pt_BR |
| Subject | Substituted quinolines | pt_BR |
| Subject | Antiviral | pt_BR |
| Subject | PBMC | pt_BR |
| DeCS | Infecções por HTLV-I/patologia | pt_BR |
| DeCS | Monócitos/efeitos de drogas | pt_BR |
| DeCS | Quinolinas/farmacologia | pt_BR |
| DeCS | Proliferação de Células/efeitos de drogas | pt_BR |
| DeCS | Sobrevivência Celular/efeitos de drogas | pt_BR |
| DeCS | Células Cultivadas | pt_BR |
| DeCS | Ensaio de Imunoadsorção Enzimática | pt_BR |
| DeCS | Humanos | pt_BR |
| DeCS | Indicadores e Reagentes | pt_BR |
| DeCS | Sais de Tetrazólio | pt_BR |
| DeCS | Azul Tripano | pt_BR |
