| Author | Bouillet, Leoneide Erica Maduro | |
| Author | Dias, Mariana Oliveira | |
| Author | Dorigo, Natália Alves | |
| Author | Moura, Andrew Douglas | |
| Author | Russell, Bruce | |
| Author | Nosten, Francois | |
| Author | Renia, Laurent | |
| Author | Braga, Erika Martins | |
| Author | Gazzinelli, Ricardo Tostes | |
| Author | Rodrigues, Mauricio Martins | |
| Author | Soares, Irene da Silva | |
| Author | Romero, Oscar Bruna | |
| Access date | 2014-08-04T18:08:15Z | |
| Available date | 2014-08-04T18:08:15Z | |
| Document date | 2011 | |
| Citation | BOUILLET, Leoneide Erica Maduro et al. Long-term humoral and cellular immune responses elicited by a heterologous Plasmodium vivax apical membrane antigen 1 protein prime/adenovirus boost immunization protocol. Infect Immun, 79(9): 3642-3652, 2011. | pt_BR |
| ISSN | 0019-9567 | |
| URI | https://www.arca.fiocruz.br/handle/icict/8122 | |
| Language | eng | pt_BR |
| Publisher | American Society for Microbiology | pt_BR |
| Rights | open access | pt_BR |
| Title | Long-term humoral and cellular immune responses elicited by a heterologous Plasmodium vivax apical membrane antigen 1 protein prime/adenovirus boost immunization protocol | pt_BR |
| Type | Article | pt_BR |
| Abstract | Apical membrane antigen 1 (AMA-1) is an invasion-related Plasmodium antigen that is expressed during both intracellular and extracellular asexual stages of the parasite’s life cycle, making it an ideal target for induction of humoral and cellular immune responses that can protect against malaria. We show here that when it is administered as a recombinant protein (P) in Montanide ISA720 adjuvant, followed by a recombinant human type 5 adenovirus (Ad), intense and long-lasting Plasmodium vivax AMA-1-specific antibody responses (including both IgG1 and IgG2a), as well as proliferative memory T cell responses, can be detected in immunized mice. Memory T cells displayed both central (CD44 hi CD62L hi ) and effector (CD44 hi CD62L lo) phenotypes, with the central memory phenotype prevailing (56% of AMA-1-specific proliferating cells). Considering the main traits of the memory immune responses induced against AMA-1, this particular sequence of immunogens (P followed by Ad), but no others (Ad/Ad, Ad/P, or P/P), displayed an optimal synergistic effect.
These results give further support to the need for preclinical studies of P. vivax vaccine candidate AMA-1 administered in prime/boost protocols that include recombinant proteins and adenoviral vectors | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Agency for Science Technology and Research. Singapore Immunology Network. Biopolis. Singapore | pt_BR |
| Affilliation | Shoklo Malaria Research Unit. Mae Sot, Tak, Thailand/Center for Clinical Vaccinology and Tropical Medicine. Churchill Hospital. Oxford, United Kingdom/Mahidol University. Faculty of Tropical Medicine. Bangkok, Thailand | pt_BR |
| Affilliation | Agency for Science Technology and Research. Singapore Immunology Network. Biopolis. Singapore | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxocologia. São Paulo, SP, Brazil | pt_BR |
| Affilliation | Universidade de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas Rene ́ Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | Plasmodium vivax | pt_BR |
| Subject | vaccines | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
