| Author | Gao, Feng | |
| Author | Morrison, Sandre G | |
| Author | Robertson, David L | |
| Author | Thornton, Charlotte L | |
| Author | Craig, Stevenson | |
| Author | Karlsson, Gunilla | |
| Author | Sodroski, Joseph | |
| Author | Morgado, Mariza Gonçalves | |
| Author | Castro Filho, Bernardo Galvão | |
| Author | Von Briesen, Hagen | |
| Author | Beddows, Simon | |
| Author | Weber, Jonathan | |
| Author | Sharp, Paul M | |
| Author | Shaw, George M | |
| Author | Hahn, Beatrice H | |
| Author | Who NIAID Networks for HIV isolation and characterization | |
| Access date | 2014-08-15T19:12:06Z | |
| Available date | 2014-08-15T19:12:06Z | |
| Document date | 1996 | |
| Citation | GAO, F. et al. Molecular cloning and analysis of functional envelope genes from human immunodeficiency virus type 1 sequence subtypes A through G. Journal of Virology, v. 70, n. 3, p. 1651-1667, 1996. | pt_BR |
| ISSN | 0022-538X | |
| URI | https://www.arca.fiocruz.br/handle/icict/8201 | |
| Language | eng | pt_BR |
| Publisher | American Society of Parasitologists | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Molecular cloning and analysis of functional envelope genes from human immunodeficiency virus type 1 sequence subtypes A through G. | pt_BR |
| Type | Article | |
| Abstract | Present knowledge of human immunodeficiency virus type 1 (HIV-1) envelope immunobiology has been derived almost exclusively from analyses of subtype B viruses, yet such viruses represent only a minority of strains currently spreading worldwide. To generate a more representative panel of genetically diverse envelope genes, we PCR amplified, cloned, and sequenced complete gp160 coding regions of 35 primary (peripheral blood mononuclear cell-propagated) HIV-1 isolates collected at major epicenters of the current AIDS pandemic. Analysis of their deduced amino acid sequences revealed several important differences from prototypic subtype B strains, including changes in the number and distribution of cysteine residues, substantial length differences in hypervariable regions, and premature truncations in the gp41 domain. Moreover, transiently expressed glycoprotein precursor molecules varied considerably in both size and carbohydrate content. Phylogenetic analyses of full-length env sequences indicated that the panel included members of all major sequence subtypes of HIV-1 group M (clades A to G), as well as an intersubtype recombinant (F/B) from an infected individual in Brazil. In addition, all subtype E and three subtype G viruses initially classified on the basis of partial env sequences were found to cluster in subtype A in the 3' half of their gp41 coding region, suggesting that they are also recombinant. The biological activity of PCR-derived env genes was examined in a single-round virus infectivity assay. This analysis identified 20 clones, including 1 from each subtype (or recombinant), which expressed fully functional envelope glycoproteins. One of these, derived from a patient with rapid CD4 cell decline, contained an amino acid substitution in a highly conserved endocytosis signal (Y721C), as mediated virus entry with very poor efficiency, although they did not contain sequence changes predicted to alter protein function. These results indicate that the env genes of primary HIV-1 isolates collected worldwide can vary considerably in their genetic, phylogenetic, and biological properties. The panel of env constructs described here should prove valuable for future structure-function studies of naturally occurring envelope glycoproteins as well as AIDS vaccine development efforts targeted against a broader spectrum of viruses. | pt_BR |
| Affilliation | University of Alabama at Birmingham. Departments of Medicine and Microbiology. Birmingham, Alabama | pt_BR |
| Affilliation | University of Alabama at Birmingham. Departments of Medicine and Microbiology. Birmingham, Alabama | pt_BR |
| Affilliation | University of Nottingham. Queens Medical Centre. Department of Genetics. Nottingham | pt_BR |
| Affilliation | University of Alabama at Birmingham. Departments of Medicine and Microbiology. Birmingham, Alabama | pt_BR |
| Affilliation | University of Alabama at Birmingham. Departments of Medicine and Microbiology. Birmingham, Alabama | pt_BR |
| Affilliation | Dana-Farber Cancer Institute. Department of Pathology. Boston, Massachusetts | pt_BR |
| Affilliation | Dana-Farber Cancer Institute. Department of Pathology. Boston, Massachusetts | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil | pt_BR |
| Affilliation | Chemotherapeutisches Forschungsinstitut. Georg Speyer Haus. Frankfurt am Main. Germany | pt_BR |
| Affilliation | St. Mary’s Hospital Medical School. Department of Communicable Diseases. Imperial College. London | pt_BR |
| Affilliation | St. Mary’s Hospital Medical School. Department of Communicable Diseases. Imperial College. London | pt_BR |
| Affilliation | University of Nottingham. Queens Medical Centre. Department of Genetics. Nottingham | pt_BR |
| Affilliation | University of Alabama at Birmingham. Departments of Medicine and Microbiology. Birmingham, Alabama | pt_BR |
| Affilliation | University of Alabama at Birmingham. Departments of Medicine and Microbiology. Birmingham, Alabama | pt_BR |
| DeCS | Produtos do Gene env/genética | pt_BR |
| DeCS | HIV-1/genética | pt_BR |
| DeCS | Sequência de Aminoácidos | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Sequência de Bases | pt_BR |
| DeCS | Clonagem Molecular | pt_BR |
| DeCS | DNA Viral | pt_BR |
| DeCS | Expressão Gênica/efeitos de drogas | pt_BR |
| DeCS | Produtos do Gene env/fisiologia | pt_BR |
| DeCS | Proteína gp120 do Envelope de HIV/genética | pt_BR |
| DeCS | Proteína gp41 do Envelope de HIV/fisiologia | pt_BR |
| DeCS | Infecções por HIV/virologia | pt_BR |
| DeCS | HIV-1/classificação | pt_BR |
| DeCS | Células HeLa | pt_BR |
| DeCS | Humanos | pt_BR |
| DeCS | Dados de Sequência Molecular | pt_BR |
| DeCS | Filogenia | pt_BR |
| DeCS | Reação em Cadeia da Polimerase | pt_BR |
| DeCS | Precursores de Proteínas/genética | pt_BR |
| DeCS | Recombinação Genética | pt_BR |
| DeCS | Tunicamicina/farmacologia | pt_BR |
