| Author | Santos, Leonardo Nascimento | |
| Author | Gaallo, Margareth Borges Coutinho | |
| Author | Silva, Eduardo Santos | |
| Author | Figueiredo, Camila Alexandrina Viana | |
| Author | Cooper, Philip John | |
| Author | Barreto, Maurício Lima | |
| Author | Loureiro, S | |
| Author | Pontes-de-Carvalho, Lain Carlos | |
| Author | Neves, Neuza Maria Alcântara | |
| Access date | 2014-08-25T16:31:26Z | |
| Available date | 2014-08-25T16:31:26Z | |
| Document date | 2013 | |
| Citation | SANTOS, L. N. et al. A proteomic approach to identify proteins from Trichuris trichiura extract with immunomodulatory effects. Parasite Immunology, v. 35, n. 5-6, p. 188-193, 2013. | pt_BR |
| ISSN | 1365-3024 | |
| URI | https://www.arca.fiocruz.br/handle/icict/8223 | |
| Language | eng | pt_BR |
| Publisher | John Wiley & Sons, Ltd | pt_BR |
| Rights | open access | pt_BR |
| Title | A proteomic approach to identify proteins from Trichuris trichiura extract with immunomodulatory effects. | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1111/pim.12025 | |
| Abstract | Infections with Trichuris trichiura and other trichurid nematodes have been reported to display protective effects against atopy, allergic and autoimmune diseases. The aims of the present study were to investigate the immunomodulatory properties of T. trichiura adult worm extract (TtE) and its fractions (TtEFs) on the production of cytokines by peripheral blood mononuclear cells and to identify their proteinaceous components. Fourteen TtEFs were obtained by ion exchange chromatography and tested for effects on cytokine production by peripheral blood mononuclear cells. The molecular constituents of the six most active fractions were evaluated using nano-LC/mass spectrometry. The homology between T. trichiura and the related nematode Trichinella spiralis was used to identify 12 proteins in TtEFs. Among those identified, fructose biphosphate aldolase, a homologue of macrophage migration inhibitory factor and heat-shock protein 70 may contribute to the immunomodulatory effects of TtEFs. The identification of such proteins could lead to the development of novel drugs for the therapy of allergic and other inflammatory diseases | pt_BR |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeração. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Plataforma de Espectrometria de Massas. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeração. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeração. Salvador, BA, Brasil | pt_BR |
| Affilliation | Liverpool School of Tropical Medicine. Department of Molecular and Biochemical Parasitology. Liverpool, UK | pt_BR |
| Affilliation | Universidade Federal da Bahia. Instituto de Saúde Coletiva. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Instituto de Saúde Coletiva. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laborat orio de Patologia e Biointervenção. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeração. Salvador, BA, Brasil | pt_BR |
| Subject | Cytokine | pt_BR |
| Subject | Immunoregulation | pt_BR |
| Subject | Peripheral blood mononuclear cells | pt_BR |
| Subject | Proteomics | pt_BR |
| Subject | Trichuris spp | pt_BR |
| DeCS | Citocinas/sangue | pt_BR |
| DeCS | Proteínas de Helminto/imunologia | pt_BR |
| DeCS | Leucócitos Mononucleares/imunologia | pt_BR |
| DeCS | Trichuris/imunologia | pt_BR |
| DeCS | Adulto | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Criança | pt_BR |
| DeCS | Cromatografia por Troca Iônica | pt_BR |
| DeCS | Frutose-Bifosfato Aldolase/química | pt_BR |
| DeCS | Proteínas de Choque Térmico HSP70/imunologia | pt_BR |
| DeCS | Proteínas de Helminto/química | pt_BR |
| DeCS | Humanos | pt_BR |
| DeCS | Proteômica | pt_BR |
| DeCS | Trichinella spiralis/química | pt_BR |
| DeCS | Tricuríase/imunologia | pt_BR |
| DeCS | Adulto Jovem | pt_BR |
