| Author | Santos, Théo de Araújo | |
| Author | Rodríguez, Nilda E | |
| Author | Pontes, Sara Moura | |
| Author | Dixt, Upasna Gaur | |
| Author | Abánades, Daniel Ruiz | |
| Author | Bozza, Patrícia T. | |
| Author | Wilson, Mary Edithe | |
| Author | Borges, Valeria de Matos | |
| Access date | 2014-09-29T16:39:00Z | |
| Available date | 2014-09-29T16:39:00Z | |
| Document date | 2014 | |
| Citation | SANTOS, T. A. et al. Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence. Journal of Infectious Diseases, jun. 2014. | pt_BR |
| ISSN | 0022-1899 | |
| URI | https://www.arca.fiocruz.br/handle/icict/8466 | |
| Language | eng | pt_BR |
| Publisher | Oxford University Press | pt_BR |
| Rights | open access | |
| Title | Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence | pt_BR |
| Type | Article | |
| DOI | 10.1093/infdis/jiu299 | |
| Abstract | Lipid bodies (LB; lipid droplets) are cytoplasmic organelles involved in lipid metabolism. Mammalian LBs display
an important role in host-pathogen interactions, but the role of parasite LBs in biosynthesis of prostaglandin
F2α (PGF2α) has not been investigated. We report herein that LBs increased in abundance during
development of Leishmania infantum chagasi to a virulent metacyclic stage, as did the expression of PGF2α synthase
(PGFS). The amount of parasite LBs and PGF2α were modulated by exogenous arachidonic acid. During
macrophage infection, LBs were restricted to parasites inside the parasitophorous vacuoles (PV). We detected
PGF2α receptor (FP) on the Leishmania PV surface. The blockage of FP with AL8810, a selective antagonist,
hampered Leishmania infection, whereas the irreversible inhibition of cyclooxygenase with aspirin increased
the parasite burden. These data demonstrate novel functions for parasite-derived LBs and PGF2α in the cellular
metabolism of Leishmania and its evasion of the host immune response. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / University of Iowa and the Iowa City VA Medical Center. Iowa City, Iowa | pt_BR |
| Affilliation | University of Iowa and the Iowa City VA Medical Center. Iowa City, Iowa | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil | pt_BR |
| Affilliation | University of Iowa and the Iowa City VA Medical Center. Iowa City, Iowa | pt_BR |
| Affilliation | Centro de Investigaciones Biológicas, CSIC. Department of Chemical and Physical Biology. Madrid, Spain | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | University of Iowa and the Iowa City VA Medical Center. Iowa City, Iowa | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Institute for Investigation in Immunology, iii-INCT. National Institute of Science and Technology. São Paulo, SP, Brasil | pt_BR |
| Subject | Leishmania infantum chagasi | pt_BR |
| Subject | Lipid droplets | pt_BR |
| Subject | Prostaglandin F2α | pt_BR |
| Subject | PGF2α synthase | pt_BR |
| Subject | Macrophage infection | pt_BR |
