Author | Soares, Milena Botelho Pereira | |
Author | Lima, Ricardo Santana de | |
Author | Souza, Bruno Solano de Freitas | |
Author | Vasconcelos, Juliana Fraga | |
Author | Rocha, Leonardo Lima | |
Author | Santos, Ricardo Ribeiro dos | |
Author | Iacobas, Sanda | |
Author | Goldenberg, Regina Coeli dos Santos | |
Author | Lisanti, Michael P | |
Author | Iacobas, Dumitru Andrei | |
Author | Tanowitz, Herbert Bernard | |
Author | Spray, David Conover | |
Author | Carvalho, Antonio Carlos Campos de | |
Access date | 2014-10-13T17:55:09Z | |
Available date | 2014-10-13T17:55:09Z | |
Document date | 2011 | |
Citation | SOARES, M. B. P. et al. Reversion of gene expression alterations in hearts of mice with chronic chagasic cardiomyopathy after transplantation of bone marrow cells. Cell Cycle, v. 10, n. 9, p. 1448-1455, 2011. | pt_BR |
ISSN | 1551-4005 | |
URI | https://www.arca.fiocruz.br/handle/icict/8579 | |
Language | eng | pt_BR |
Publisher | Landes Bioscience | pt_BR |
Rights | open access | pt_BR |
Title | Reversion of gene expression alterations in hearts of mice with chronic chagasic cardiomyopathy after transplantation of bone marrow cells. | pt_BR |
Type | Article | pt_BR |
DOI | 10.4161/cc.10.9.15487 | |
Abstract | Chronic chagasic cardiomyopathy is a leading cause of heart failure in Latin American countries, being associated with intense inflammatory response and fibrosis. We have previously shown that bone marrow mononuclear cell (BMC) transplantation improves inflammation, fibrosis, and ventricular diameter in hearts of mice with chronic Chagas disease. Here we investigated the transcriptomic recovery induced by BMC therapy by comparing the heart transcriptomes of control, chagasic, and BMC transplanted mice. Out of the 9390 unique genes quantified in all samples, 1702 had their expression altered in chronic chagasic hearts compared to those of normal mice. Major categories of significantly upregulated genes were related to inflammation, fibrosis and immune responses, while genes involved in mitochondrion function were downregulated. When BMC-treated chagasic hearts were compared to infected mice, 96% of the alterations detected in infected hearts were restored to normal levels, although an additional 109 genes were altered by treatment. Transcriptomic recovery, a new measure that considers both resotrative and side effects of treatment, was remarkably high (84%). Immunofluorescence and morphometric analyses confirmed the effects of BMC therapy in the pattern of inflammatory-immune response and expression of adhesion molecules. In conclusion, by using large-scale gene profiling for unbiased assessment of therapeutic efficacy we demonstrate immunomodulatory effects of BMC therapy in chronic chagasic cardiomyopathy and identify potentially relevant factors involved in the pathogenesis of the disease that may provide new therapeutic targets | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal do Vale do São Francisco. Departamento de Medicina. Petrolina, PE, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil | pt_BR |
Affilliation | Albert Einstein College of Medicine. Dominick P. Purpura Department of Neuroscience. Bronx, NY, USA | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Thomas Jefferson University. Department of Stem Cell Biology and Regenerative Medicine. Kimmel Cancer Center. Philadelphia, PA, USA | pt_BR |
Affilliation | Albert Einstein College of Medicine. Dominick P. Purpura Department of Neuroscience. Bronx, NY, USA | pt_BR |
Affilliation | Albert Einstein College of Medicine. Department of Medicine. Bronx, NY, USA / Albert Einstein College of Medicine. Department of Pathology. Bronx, NY, USA | pt_BR |
Affilliation | Albert Einstein College of Medicine. Dominick P. Purpura Department of Neuroscience. Bronx, NY, USA / Albert Einstein College of Medicine. Department of Medicine. Bronx, NY, USA | pt_BR |
Affilliation | Albert Einstein College of Medicine. Dominick P. Purpura Department of Neuroscience. Bronx, NY, USA / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / 8Instituto Nacional de Cardiologia. Rio de Janeiro, RJ, Brasil | pt_BR |
Subject | Chagas disease | pt_BR |
Subject | Trypanosoma cruzi | pt_BR |
Subject | Cardiomyopathy | pt_BR |
Subject | Cell transplantation | pt_BR |
Subject | Microarray | pt_BR |
Subject | Inflammation | pt_BR |
Subject | Fibrosis | pt_BR |
DeCS | Transplante de Medula Óssea/patologia | pt_BR |
DeCS | Cardiomiopatia Chagásica/genética | pt_BR |
DeCS | Regulação da Expressão Gênica/imunologia | pt_BR |
DeCS | Miocárdio/imunologia | pt_BR |
DeCS | Trypanosoma cruzi/imunologia | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Transplante de Medula Óssea/imunologia | pt_BR |
DeCS | Cardiomiopatia Chagásica/imunologia | pt_BR |
DeCS | Cardiomiopatia Chagásica/terapia | pt_BR |
DeCS | Doença Crônica | pt_BR |
DeCS | Modelos Animais de Doenças | pt_BR |
DeCS | Feminino | pt_BR |
DeCS | Fibrose | pt_BR |
DeCS | Galectina 3/genética | pt_BR |
DeCS | Perfilação da Expressão Gênica/métodos | pt_BR |
DeCS | Masculino | pt_BR |
DeCS | Camundongos | pt_BR |
DeCS | Camundongos Endogâmicos C57BL | pt_BR |
DeCS | Miocárdio/metabolismo | pt_BR |
DeCS | Análise de Sequência com Séries de Oligonucleotídeos | pt_BR |
DeCS | Trypanosoma cruzi/classificação | pt_BR |
DeCS | Fator de von Willebrand/genética | pt_BR |