Author | Moens, Britta | |
Author | Pannecouque, Christophe | |
Author | López, Giovanni | |
Author | Talledo, Michael | |
Author | Gotuzzo, Eduardo | |
Author | Cunha, Antonio Ricardo Khouri | |
Author | Bittencourt, Achilea Candida Lisboa | |
Author | Vallve, Maria de Lourdes Farre | |
Author | Castro Filho, Bernardo Galvão | |
Author | Vandamme, Anne-Mieke | |
Author | Van Weyenbergh, Johan Jozef Rosa Maria | |
Access date | 2014-11-11T19:09:55Z | |
Available date | 2014-11-11T19:09:55Z | |
Document date | 2012 | |
Citation | MOENS, B. et al. Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines. Virology Journal, v. 9, p. 171, 2012. | pt_BR |
ISSN | 1743-422X | |
URI | https://www.arca.fiocruz.br/handle/icict/8793 | |
Language | eng | pt_BR |
Publisher | BioMed Central | pt_BR |
Rights | open access | pt_BR |
Title | Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines. | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/1743-422X-9-171 | |
Abstract | BACKGROUND: IFN-α contributes extensively to host immune response upon viral infection through antiviral, pro-apoptotic, antiproliferative and immunomodulatory activities. Although extensively documented in various types of human cancers and viral infections, controversy exists in the exact mechanism of action of IFN-α in human immunodeficiency virus type 1 (HIV-1) and human T-lymphotropic virus type 1 (HTLV-1) retroviral infections. RESULTS: IFN-α displayed strong anti-HIV-1 effects in HIV-1/HTLV-1 co-infected MT-4 cells in vitro, demonstrated by the dose-dependent inhibition of the HIV-1-induced cytopathic effect (IC50 = 83.5 IU/ml, p < 0.0001) and p24 levels in cell-free supernatant (IC50 = 1.2 IU/ml, p < 0.0001). In contrast, IFN-α treatment did not affect cell viability or HTLV-1 viral mRNA levels in HTLV-1 mono-infected cell lines, based on flow cytometry and nCounter analysis, respectively. However, we were able to confirm the previously described post-transcriptional inhibition of HTLV-1 p19 secretion by IFN-α in cell lines (p = 0.0045), and extend this finding to primary Adult T cell Leukemia patient samples (p = 0.031). In addition, through microarray and nCounter analysis, we performed the first genome-wide simultaneous quantification of complete human and retroviral transciptomes, demonstrating significant transcriptional activation of interferon-stimulated genes without concomitant decrease of HTLV-1 mRNA levels. CONCLUSIONS: Taken together, our results indicate that both the absence of in vitro antiproliferative and pro-apoptotic activity as well as the modest post-transcriptional antiviral activity of IFN-α against HTLV-1, were not due to a cell-intrinsic defect in IFN-α signalisation, but rather represents a retrovirus-specific phenomenon, considering the strong HIV-1 inhibition in co-infected cells. | pt_BR |
Affilliation | Rega Institute for Medical Research,Laboratory of Clinical and Epidemiological Virology K.U.Leuven. Leuven, Belgium | pt_BR |
Affilliation | Rega Institute for Medical Research. Laboratory for Virology and Chemotherapy K.U.Leuven. Leuven, Belgium | pt_BR |
Affilliation | Universidad Peruana Cayetano Heredia. Instituto de Medicina Tropical Alexander von Humboldt. Lima, Peru | pt_BR |
Affilliation | Universidad Peruana Cayetano Heredia. Instituto de Medicina Tropical Alexander von Humboldt. Lima, Peru | pt_BR |
Affilliation | Universidad Peruana Cayetano Heredia. Instituto de Medicina Tropical Alexander von Humboldt. Lima, Peru / Universidad Peruana Cayetano Heredia. Facultad de Medicina. Departamento de Medicina. Lima, Peru | pt_BR |
Affilliation | Rega Institute for Medical Research,Laboratory of Clinical and Epidemiological Virology K.U.Leuven. Leuven, Belgium / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Federal University of Bahia. Professor Edgar Santos Teaching Hospital. Department of Pathology. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Bahia School of Medicine and Public Health. Salvador, BA, Brasil | pt_BR |
Affilliation | Rega Institute for Medical Research,Laboratory of Clinical and Epidemiological Virology K.U.Leuven. Leuven, Belgium / Universidade Nova de Lisboa. Instituto de Higiene e Medicina Tropical. Centro de Malária e outras Doenças Tropicais. Lisboa, Portugal | pt_BR |
Affilliation | Rega Institute for Medical Research,Laboratory of Clinical and Epidemiological Virology K.U.Leuven. Leuven, Belgium / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Institute for Immunological Investigation iii-INCT. São Paulo, SP, Brasil | pt_BR |
Subject | Retrovirus | pt_BR |
Subject | IFN-α | pt_BR |
Subject | HIV-1 | pt_BR |
Subject | HTLV-1 | pt_BR |
Subject | IFN-α signaling | pt_BR |
Subject | Antiviral activity | pt_BR |
DeCS | Linfócitos T CD4-Positivos/imunologia | pt_BR |
DeCS | Linfócitos T CD4-Positivos/virologia | pt_BR |
DeCS | Vírus 1 Linfotrópico T Humano/imunologia | pt_BR |
DeCS | Interferon-alfa/biossíntese | pt_BR |
DeCS | Transdução de Sinal | pt_BR |
DeCS | Sobrevivência Celular | pt_BR |
DeCS | Perfilação da Expressão Gênica | pt_BR |
DeCS | HIV-1/imunologia | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | RNA/biossíntese | pt_BR |
DeCS | RNA/genética | pt_BR |