Author | Martins, Gustavo Ferreira | |
Author | Ortigão, José Marcelo Ramalho | |
Author | Lobo, Neil Francis | |
Author | Severson, David William | |
Author | McDowel, Mary Ann | |
Author | Pimenta, Paulo Filemon Paolucci | |
Access date | 2014-11-21T14:36:12Z | |
Available date | 2014-11-21T14:36:12Z | |
Document date | 2011 | |
Citation | MARTINS, Gustavo Ferreira et al. Insights into the transcriptome of oenocytes from Aedes aegypti pupae. Memorias do Instituto Oswaldo Cruz, v. 106, n. 3 p. 308-315, 2011. | pt_BR |
ISSN | 0074-0276 | |
URI | https://www.arca.fiocruz.br/handle/icict/8936 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz. | pt_BR |
Rights | open access | pt_BR |
Title | Insights into the transcriptome of oenocytes from Aedes aegypti pupae. | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/S0074-02762011000300009 | |
Abstract | Oenocytes are ectodermic cells present in the fat body of several insect species and these cells are considered to be analogous to the mammalian liver, based on their role in lipid storage, metabolism and secretion. Although oenocytes were identified over a century ago, little is known about their messenger RNA expression profiles. In this study, we investigated the transcriptome of Aedes aegypti oenocytes. We constructed a cDNA library from Ae. aegypti MOYO-R strain oenocytes collected from pupae and randomly sequenced 687 clones. After sequences editing and assembly, 326 high-quality contigs were generated. The most abundant transcripts identified corresponded to the cytochrome P450 superfamily, whose members have roles primarily related to detoxification and lipid metabolism. In addition, we identified 18 other transcripts with putative functions associated with lipid metabolism. One such transcript, a fatty acid synthase, is highly represented in the cDNA library of oenocytes. Moreover, oenocytes expressed several immunity-related genes and the majority of these genes were lysozymes. The transcriptional profile suggests that oenocytes play diverse roles, such as detoxification and lipid metabolism, and increase our understanding of the importance of oenocytes in Ae. aegypti homeostasis and immune competence | pt_BR |
Affilliation | Universidade Federal de Viçosa. Departamento de Biologia Geral. Viçosa, MG, Brasil | pt_BR |
Affilliation | Kansas State University. Department of Entomology. Manhattan, Kansas, USA | pt_BR |
Affilliation | University of Notre Dame. Center for Global Health and Infectious Diseases. Department of Biological Sciences. Notre Dame, IN, USA | pt_BR |
Affilliation | University of Notre Dame. Center for Global Health and Infectious Diseases. Department of Biological Sciences. Notre Dame, IN, USA | pt_BR |
Affilliation | University of Notre Dame. Center for Global Health and Infectious Diseases. Department of Biological Sciences. Notre Dame, IN, USA | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brasil | pt_BR |
Subject | Aedes aegypti | pt_BR |
Subject | oenocytes | pt_BR |
Subject | transcriptome | pt_BR |
Subject | cytochrome P450 | pt_BR |
Subject | lipid metabolism | pt_BR |
Subject | detoxification | pt_BR |