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AutorSoares, Emanoella L.
AutorShah, Mohibullah
AutorSoares, Arlete A.
AutorCosta, José H.
AutorCarvalho, Paulo Costa
AutorDomont, Gilberto B.
AutorNogueira, Fábio Cesar Sousa
AutorCampos, Francisco A. P.
Fecha de acceso2014-11-24T12:13:37Z
Fecha de disponibilización2015-08-01T07:30:05Z
Fecha de publicación2014
ReferenciaSOARES, Emanoella L. et al. Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds. Journal of Proteome Research, v. 13, n. 8, p. 3562-3570, 2014.pt_BR
ISSN1535-3893
URIhttps://www.arca.fiocruz.br/handle/icict/8942
Idiomaengpt_BR
EditorJournal of Proteome Researchpt_BR
Derechos de autoropen accesspt_BR
TítuloProteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seedspt_BR
Tipo del documentoArticlept_BR
DOI10.1021/pr5004505pt_BR
Resumen en InglésIn this study, we performed a systematic proteomic analysis of the inner integument from developing seeds of Jatropha curcas and further explored the protein machinery responsible for generating the carbon and nitrogen sources to feed the growing embryo and endosperm. The inner integument of developing seeds was dissected into two sections called distal and proximal, and proteins were extracted from these sections and from the whole integument and analyzed using an EASY-nanoLC system coupled to an ESILTQ-Orbitrap Velos mass spectrometer. We identified 1526, 1192, and 1062 proteins from the proximal, distal, and whole inner integuments, respectively. The identifications include those of peptidases and other hydrolytic enzymes that play a key role in developmental programmed cell death and proteins associated with the cell-wall architecture and modification. Because many of these proteins are differentially expressed within the integument cell layers, these findings suggest that the cells mobilize an array of hydrolases to produce carbon and nitrogen sources from proteins, carbohydrates, and lipids available within the cells. Not least, the identification of several classes of seed storage proteins in the inner integument provides additional evidence of the role of the seed coat as a transient source of reserves for the growing embryo and endosperm.pt_BR
AfiliaciónFederal University of Ceara. Department of Biochemistry and Molecular Biology. Fortaleza, CE, Brazil.pt_BR
AfiliaciónFederal University of Ceara. Department of Biochemistry and Molecular Biology. Fortaleza, CE, Brazil.pt_BR
AfiliaciónFederal University of Ceara. Department of Biology. Campus do Pici, Fortaleza, CE, Brazil.pt_BR
AfiliaciónFederal University of Ceara. Department of Biochemistry and Molecular Biology. Fortaleza, CE, Brazil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, Brasil.pt_BR
AfiliaciónFederal University of Rio de Janeiro. Proteomic Unit, Institute of Chemistry. Rio de Janeiro, RJ, Brazil.pt_BR
AfiliaciónFederal University of Rio de Janeiro. Proteomic Unit, Institute of Chemistry. Rio de Janeiro, RJ, Brazil.pt_BR
AfiliaciónFederal University of Ceara. Department of Biochemistry and Molecular Biology. Fortaleza, CE, Brazil.pt_BR
Palavras clave en InglêsLabel-free quantitative proteomicspt_BR
Palavras clave en InglêsProgrammed cell deathpt_BR
Palavras clave en InglêsJatropha curcaspt_BR
Palavras clave en InglêsOilseedpt_BR
Palavras clave en InglêsSeed coatpt_BR
Fecha de embargo2015-07-31


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