| Author | Mendes, Érica Araújo | |
| Author | Caetano, Braulia Costa | |
| Author | Penido, Marcus Luiz de Oliveira | |
| Author | Bruna-Romero, Oscar | |
| Author | Gazzinelli, Ricardo Tostes | |
| Access date | 2014-11-24T17:49:07Z | |
| Available date | 2014-11-24T17:49:07Z | |
| Document date | 2011 | |
| Citation | MENDES, Érica Araújo et al. MyD88-dependent protective immunity elicited by adenovirus 5 expressing the surface antigen 1 from Toxoplasma gondii is mediated by CD8(+) T lymphocytes. Vaccine, v. 29, n.27 p.4476-4484, 2011. | pt_BR |
| ISSN | 0264-410X | |
| URI | https://www.arca.fiocruz.br/handle/icict/8951 | |
| Language | eng | pt_BR |
| Publisher | Elsevier Science | pt_BR |
| Rights | open access | pt_BR |
| Title | MyD88-dependent protective immunity elicited by adenovirus 5 expressing the surface antigen 1 from Toxoplasma gondii is mediated by CD8(+) T lymphocytes | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.vaccine.2011.04.044 | |
| Abstract | Toxoplasma gondii is an intracellular parasite widely spread around the world. The Surface Antigens (SAG) 1, 2 and 3 are the main proteins expressed on the surface of T. gondii tachyzoites. Replication-defective adenovirus serotype 5 (rAd5) is one of the most potent recombinant viral vectors for eliciting T cell-mediated immunity in mice and humans. Here we show that vaccination with rAd5 expressing SAG1 (AdSAG1), but neither SAG2 nor SAG3, induces protective immunity in the highly susceptible C57BL/6 mice challenged with T. gondii. Furthermore, we evaluated different immunological components involved on viral induced protective immunity. We observed that host protection elicited by AdSAG1 is highly dependent on IL-12, IFN-γ and CD8+ T lymphocytes. Importantly, the induction of protective immunity (T cell-derived IFN-γ) was also dependent on Myeloid Differentiation Factor 88 (MyD88), and thus, likely to involve Toll-Like receptors. We conclude that protective parasite specific-CD8+ T cells are elicited by a mechanism that involves MyD88-dependent induction of IL-12. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | University of Massachusetts Medical School. Division of Infectious Disease and Immunology. Worcester, MA, USA | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil/University of Massachusetts Medical School. Division of Infectious Disease and Immunology. Worcester, MA, USA/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil | pt_BR |
| Subject | Adenoviridae/metabolism | pt_BR |
| Subject | Myeloid Differentiation | pt_BR |
| Subject | Factor 88/immunology | pt_BR |
| Subject | Toxoplasmosis | pt_BR |
| Subject | Animal/parasitology | pt_BR |
| Subject | Protozoan Vaccines | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
