Author | Mendes, Rita Gabriela Pedrosa Ribeiro | |
Author | Gusmão, Michélia Antônia do Nascimento | |
Author | Maia, Ana Carolina Ribeiro Gomes | |
Author | Detoni, Michelle de Lima | |
Author | Porcino, Gabriane Nascimento | |
Author | Soares, Thais Vieira | |
Author | Juliano, Maria Aparecida | |
Author | Juliano, Luiz | |
Author | Coelho, Paulo Marcos Zech | |
Author | Lenzi, Henrique Leonel | |
Author | Pinto, Priscila de Faria | |
Author | Vasconcelos, Eveline Gomes | |
Access date | 2014-11-24T18:01:13Z | |
Available date | 2014-11-24T18:01:13Z | |
Document date | 2011 | |
Citation | MENDES, Rita Gabriela Pedrosa Ribeiro et al. Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro¬lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 106, n. 7, p. 808-813, 2011. | pt_BR |
ISSN | 0074-0276 | |
URI | https://www.arca.fiocruz.br/handle/icict/8953 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz | pt_BR |
Rights | open access | pt_BR |
Title | Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro¬lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/S0074-02762011000700005 | |
Abstract | A peptide (SmB2LJ; r175-194) that belongs to a conserved domain from Schistosoma mansoni SmATPDase 2 and is shared with potato apyrase, as predicted by in silico analysis as antigenic, was synthesised and its immunostimulatory property was analysed. When inoculated in BALB/c mice, this peptide induced high levels of SmB2LJ-specific IgG1 and IgG2a subtypes, as detected by enzyme linked immunosorbent assay. In addition, dot blots were found to be positive for immune sera against potato apyrase and SmB2LJ. These results suggest that the conserved domain r175-194 from the S. mansoni SmATPDase 2 is antigenic. Western blots were performed and the anti-SmB2LJ antibody recognised in adult worm (soluble worm antigen preparation) or soluble egg antigen antigenic preparations two bands of approximately 63 and 55 kDa, molecular masses similar to those predicted for adult worm SmATPDase 2. This finding strongly suggests the expression of this same isoform in S. mansoni eggs. To assess localisation of SmATPDase 2, confocal fluorescence microscopy was performed using cryostat sections of infected mouse liver and polyclonal antiserum against SmB2LJ. Positive reactions were identified on the external surface from the miracidium in von Lichtenberg's envelope and, in the outer side of the egg-shell, showing that this soluble isoform is secreted from the S. mansoni eggs | pt_BR |
Affilliation | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Biofísica. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Biofísica. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Biofísica. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil. | pt_BR |
Subject | Schistosoma mansoni | pt_BR |
Subject | NTPDase | pt_BR |
Subject | potato apyrase | pt_BR |
Subject | peptide | pt_BR |