Author | Campos-Junior, Paulo Henrique de Almeida | |
Author | Silva, Cristina Aragão | |
Author | Grazia, Joao Gabriel Viana | |
Author | Soares, Milena Botelho Pereira | |
Author | Santos, Ricardo Ribeiro dos | |
Author | Viana, Joao Henrique Moreira | |
Access date | 2014-11-27T17:08:27Z | |
Available date | 2014-11-27T17:08:27Z | |
Document date | 2011 | |
Citation | CAMPOS JUNIOR, P. H. A. et al. Use of ultrasound biomicroscopy to evaluate induced ovarian follicular growth and ovulation in mice. Laboratory Animals, v. 45, n. 4, p. 254-258, 2011. | pt_BR |
ISSN | 1758-1117 | |
URI | https://www.arca.fiocruz.br/handle/icict/8983 | |
Language | eng | pt_BR |
Publisher | Nature Publishing Group | pt_BR |
Rights | open access | pt_BR |
Title | Use of ultrasound biomicroscopy to evaluate induced ovarian follicular growth and ovulation in mice. | pt_BR |
Type | Article | pt_BR |
DOI | 10.1258/la.2011.011031 | |
Abstract | Recent advances in image technology, including significant gains in spatial resolution, have made realtime sequential
ovarian evaluations possible in small rodents, allowing longitudinal (continued) studies of the ovarian cycle and reducing
the required number of experimental animals. The aim of this study was to evaluate exogenous stimulated follicular growth
in mice using high-resolution ultrasound technology. Female mice (n ¼ 15) received a 5 IU intraperitoneal injection of equine
chorionic gonadotropin (eCG) and 48 h later a 5 IU injection of human chorionic gonadotropin (hCG), and were allowed to mate
thereafter. In experiment 1, animals (n ¼ 7) were evaluated every 6 h, from 3 to 51 h after eCG injection, with an ultrasound
biomicroscopy (UBM) equipped with a realtime 45 MHz microvisualization probe (RMV 707b). The ovaries were identified and
follicular population quantified, and follicles were classified according to the diameter as small ( 449 mm) or large ( 450 mm).
A significant change in the distribution of follicle population according to category was observed only 45 h after eCG injection
(P, 0.05). In experiment 2, animals (n ¼ 8) were evaluated every 2 h, from 2 h to 10 h after hCG treatment. The largest
follicles reached a maximum size (596.7+106.0 mm) 5.8+2.3 h after hCG injection. As expected, the population of large
follicles decreased thereafter, indicating the progress of ovulations, but large follicles were still detected late after treatment
(10.1+1.1 h). In conclusion, UBM can be used to evaluate follicle dynamics in superstimulated mice (C57BL/6 and BALB/c);
significant changes in follicle distribution only occur at later stages after eCG stimulation; and hCG-induced ovulations may
not occur synchronously in mice. | pt_BR |
Affilliation | Federal University of Minas Gerais. Belo Horizonte, MG, Brasil / Embrapa Dairy Cattle Research Center. Juiz de Fora, MG, Brasil | pt_BR |
Affilliation | Biotechnology and Cellular Therapy Center. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Federal University of Juiz de Fora. Juiz de Fora, MG, Brasil | pt_BR |
Affilliation | Biotechnology and Cellular Therapy Center. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Biotechnology and Cellular Therapy Center. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Embrapa Dairy Cattle Research Center. Juiz de Fora, MG, Brasil | pt_BR |
Subject | Follicular dynamics | pt_BR |
Subject | Folliculogenesis | pt_BR |
Subject | Ultrasonography | pt_BR |
Subject | Small rodents | pt_BR |
DeCS | Microscopia Acústica/métodos | pt_BR |
DeCS | Folículo Ovariano/crescimento & desenvolvimento | pt_BR |
DeCS | Folículo Ovariano/ultrassonografia | pt_BR |
DeCS | Ovulação/fisiologia | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Gonadotropina Coriônica/administração & dosagem | pt_BR |
DeCS | Feminino | pt_BR |
DeCS | Camundongos | pt_BR |
DeCS | Camundongos Endogâmicos BALB C | pt_BR |
DeCS | Fatores de Tempo | pt_BR |