| Author | Montalvão, Fabricio | |
| Author | Almeida, Geisy Monteiro | |
| Author | Silva, Elisabeth Martins da | |
| Author | Borges, Valeria de Matos | |
| Author | Vasconcellos, Rita de Cássia dos Santos | |
| Author | Takiya, Christina Maeda | |
| Author | Lopes, Marcela de Freitas | |
| Author | Nunes, Marise Pinheiro | |
| Author | DosReis, George Alexandre | |
| Access date | 2015-02-09T17:40:06Z | |
| Available date | 2015-02-09T17:40:06Z | |
| Document date | 2010 | |
| Citation | MONTALVÃO, F. et al. Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-alpha secretion and reduce parasite replication in macrophages. European Journal of Immunology, v. 40, n. 2, p. 417-425, 2010. | pt_BR |
| ISSN | 1521-4141 | |
| URI | https://www.arca.fiocruz.br/handle/icict/9464 | |
| Language | eng | pt_BR |
| Publisher | Wiley-VCH Verlag GmbH & Co. KGaA | pt_BR |
| Rights | open access | pt_BR |
| Title | Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-alpha secretion and reduce parasite replication in macrophages. | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1002/eji.200939606 | |
| Abstract | Phagocytic removal of apoptotic lymphocytes exacerbates replication of Trypanosoma cruzi in macrophages. We investigated the presence of Ab against apoptotic lymphocytes in T. cruzi infection and the role of these Ab in parasite replication. Both control and chagasic serum contained IgG Ab that opsonized apoptotic lymphocytes. Treatment of apoptotic lymphocytes with purified IgG from chagasic, but not control serum, reduced T. cruzi replication in macrophages. The protective effect of chagasic IgG depended on Fcgamma receptors, as demonstrated by the requirement for the intact Fc portion of IgG, and the effect could be abrogated by treating macrophages with an anti-CD16/CD32 Fab fragment. Chagasic IgG displayed increased reactivity against a subset of apoptotic cell Ag, as measured by flow cytometry and immunoblot analyses. Apoptotic lymphocytes treated with chagasic IgG, but not control IgG, increased production of TNF-alpha, while decreasing production of TGF-beta1 by infected macrophages. Increased control of parasite replication required TNF-alpha production. Previous immunization with apoptotic cells or injection of apoptotic cells opsonized with chagasic IgG reduced parasitemia in infected mice. These results indicate that Ab raised against apoptotic cells could play a protective role in control of T. cruzi replication by macrophages. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto Biomédico. Niterói, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto de Biologia. Niterói, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Saúde e Ambiente na Região Amazônica. Conselho Nacional de Desenvolvimento Científico e Tecnológico. Amazonas, MA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Saúde e Ambiente na Região Amazônica. Conselho Nacional de Desenvolvimento Científico e Tecnológico. Amazonas, MA, Brasil | pt_BR |
| Subject | Apoptosis | pt_BR |
| Subject | Fc receptors | pt_BR |
| Subject | Phagocytosis | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| DeCS | Anticorpos Antiprotozoários/imunologia | pt_BR |
| DeCS | Doença de Chagas/imunologia | pt_BR |
| DeCS | Linfócitos/imunologia | pt_BR |
| DeCS | Macrófagos/imunologia | pt_BR |
| DeCS | Trypanosoma cruzi/imunologia | pt_BR |
| DeCS | Fator de Necrose Tumoral alfa/secreção | pt_BR |
| DeCS | Transferência Adotiva | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Anticorpos Antiprotozoários/farmacologia | pt_BR |
| DeCS | Apoptose | pt_BR |
| DeCS | Células Cultivadas | pt_BR |
| DeCS | Doença de Chagas/parasitologia | pt_BR |
| DeCS | Técnicas de Cocultura | pt_BR |
| DeCS | Citometria de Fluxo | pt_BR |
| DeCS | Imunoglobulina G/imunologia | pt_BR |
| DeCS | Linfócitos/citologia | pt_BR |
| DeCS | Macrófagos/citologia | pt_BR |
| DeCS | Masculino | pt_BR |
| DeCS | Camundongos | pt_BR |
| DeCS | Parasitemia/imunologia | pt_BR |
| DeCS | Fator de Crescimento Transformador beta1/metabolismo | pt_BR |
| DeCS | Trypanosoma cruzi/efeitos de drogas | pt_BR |
