| Author | Silva, Patricia Costa Lima da | |
| Author | Scholte, Larissa Lopes Silva | |
| Author | Reis, Mariana Paula | |
| Author | Chaves, Anderson Vieira | |
| Author | Oliveira, Pollyanna Lacerda de | |
| Author | Itabayana, Luiza Bastani | |
| Author | Suhadolnik, Maria Luiza | |
| Author | Barbosa, Francisco Antonio Rodrigues | |
| Author | Souza, Edmar Chartone de | |
| Author | Nascimento, Andrea Maria Amaral | |
| Access date | 2015-02-13T13:08:05Z | |
| Available date | 2015-02-13T13:08:05Z | |
| Document date | 2014 | |
| Citation | SILVA, Patricia Costa Lima da, et al. Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining. PLoS ONE, v. 9, n. 4, p. e95655, 2014. | pt_BR |
| ISSN | 1553-7366 | |
| URI | https://www.arca.fiocruz.br/handle/icict/9496 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | pt_BR |
| Title | Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1371/journal.pone.0095655 | |
| Abstract | The bacterial community and genes involved in geobiocycling of arsenic (As) from sediment impacted by long-term gold mining were characterized through culture-based analysis of As-transforming bacteria and metagenomic studies of the arsC, arrA, and aioA genes. Sediment was collected from the historically gold mining impacted Mina stream, located in one of the world’s largest mining regions known as the “Iron Quadrangle”. A total of 123 As-resistant bacteria were recovered from the enrichment cultures, which were phenotypically and genotypically characterized for As-transformation. A diverse As-resistant bacteria community was found through phylogenetic analyses of the 16S rRNA gene. Bacterial isolates were affiliated with Proteobacteria, Firmicutes, and Actinobacteria and were represented by 20 genera. Most were AsV-reducing (72%), whereas AsIII-oxidizing accounted for 20%. Bacteria harboring the arsC gene predominated (85%), followed by aioA (20%) and arrA (7%). Additionally, we identified two novel As-transforming genera, Thermomonas and Pannonibacter. Metagenomic analysis of arsC, aioA, and arrA sequences confirmed the presence of these genes, with arrA sequences being more closely related to uncultured organisms. Evolutionary analyses revealed high genetic similarity between some arsC and aioA sequences obtained from isolates and clone libraries, suggesting that those isolates may represent environmentally important bacteria acting in As speciation. In addition, our findings show that the diversity ofarrA genes is wider than earlier described, once none arrA-OTUs were affiliated with known reference strains. Therefore, the molecular diversity of arrA genes is far from being fully explored deserving further attention. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biolo gicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundaçao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Grupo de Genomica e Biologia Computacional. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | DNA sequence analysis | pt_BR |
| Subject | Sequence analysis | pt_BR |
| Subject | Phylogenetics | pt_BR |
| Subject | Sequence databases | pt_BR |
| Subject | Phylogenetic analysis | pt_BR |
| Subject | Ribosomal RNA | pt_BR |
| Subject | Sequence alignment | pt_BR |
