| Author | Queto, Tulio | |
| Author | Gaspar-Elsas, Maria I. | |
| Author | Masid-de-Brito, Daniela | |
| Author | Vasconcelos, Zilton Farias Meira de | |
| Author | Ferraris, Fausto K. | |
| Author | Penido, Carmen | |
| Author | Cunha, Fernando Q. | |
| Author | Kanaoka, Yoshihide | |
| Author | Lam, Bing K. | |
| Author | Xavier-Elsas, Pedro Paulo | |
| Access date | 2015-02-26T12:46:24Z | |
| Available date | 2015-02-26T12:46:24Z | |
| Document date | 2010 | |
| Citation | QUETO, Tulio et al. Cysteinyl-leukotriene type 1 receptors transduce a critical signal for the up-regulation of eosinophilopoiesis by interleukin-13 and eotaxin in murine bone marrow. Journal of Leukocyte Biology, Winston-Salem, v. 87, p. 885-893, May 2010. | pt_BR |
| ISSN | 0741-5400 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/9546 | |
| Language | eng | pt_BR |
| Publisher | Society for Leukocyte Biology | pt_BR |
| Rights | restricted access | |
| Title | Cysteinyl-leukotriene type 1 receptors transduce a critical signal for the up-regulation of eosinophilopoiesis by interleukin-13 and eotaxin in murine bone marrow | pt_BR |
| Type | Article | |
| DOI | 10.1189/jlb.1108709 | |
| Abstract | IL-13 and eotaxin play important, inter-related roles in asthma models. In the lungs, CysLT, produced by the 5-LO-LTC4S pathway, mediate some local responses to IL-13 and eotaxin; in bone marrow, CysLT enhance IL-5-dependent eosinophil differentiation. We examined the effects of IL-13 and eotaxin on eosinophil differenti-ation. Semi-solid or liquid cultures were established from murine bone marrow with GM-CSF or IL-5, re-spectively, and the effects of IL-13, eotaxin, or CysLT on eosinophil colony formation and on eosinophil differ-entiation in liquid culture were evaluated, in the ab-sence or presence of: a) the 5-LO inhibitor zileuton, the FLAP inhibitor MK886, or the CysLT1R antagonists, montelukast and MK571; b) mutations that inactivate 5-LO, LTC4S, or CysLT1R; and c) neutralizing mAb against eotaxin and its CCR3 receptor. Both cytokines enhanced GM-CSF-dependent eosinophil colony forma-tion and IL-5-stimulated eosinophil differentiation. Al-though IL-13 did not induce eotaxin production, its ef-fects were abolished by anti-eotaxin and anti-CCR3 an-tibodies, suggesting up-regulation by IL-13 of responses to endogenous eotaxin. Anti-CCR3 blocked eotaxin completely. The effects of both cytokines were prevented by zileuton, MK886, montelukast, and MK571, as well as by inactivation of the genes coding for 5-LO, LTC4S, and CysLT1R. In the absence of either cytokine, these treatments or mutations had no effect. These findings provide evidence for: a) a novel role of eotaxin and IL-13 in regulating eosinophilopoiesis; and b) a role for CysLTRs in bone marrow cells in transduc-ing cytokine regulatory signals. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pediatria. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pediatria. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pediatria. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pediatria. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Farmacologia. Ribeirão Preto, SP, Brasil. | pt_BR |
| Affilliation | Brigham and Women’s Hospital. Department of Medicine. Division of Rheumatology. Boston, Massachusetts, USA. | pt_BR |
| Affilliation | Brigham and Women’s Hospital. Department of Medicine. Division of Rheumatology. Boston, Massachusetts, USA. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Secreted Regulatory Products | pt_BR |
| Subject | Lipid Mediators | pt_BR |
| Subject | Signaling Cascade | pt_BR |
