Author | Wildner, Letícia Muraro | |
Author | Bazzo, Maria Luiza | |
Author | Liedke, Susie Coutinho | |
Author | Nogueira, Christiane Lourenço | |
Author | Segat, Gabriela | |
Author | Senna, Simone Gonçalves | |
Author | Schlindwein, Aline Daiane | |
Author | Oliveira, Jaquelline Germano de | |
Author | Rovaris, Darcita Bürger | |
Author | Bonjardim, Claudio Antonio | |
Author | Kroon, Erna Gessien | |
Author | Ferreira, Paulo César Peregrino | |
Access date | 2015-02-27T12:56:22Z | |
Available date | 2015-02-27T12:56:22Z | |
Document date | 2014 | |
Citation | WILDNER, Letícia Muraro et al . Mycobacteria mobility shift assay: a method for the rapid identification of Mycobacterium tuberculosis and nontuberculous mycobacteria.Mem. Inst. Oswaldo Cruz, Rio de Janeiro , v. 109, n. 3, May 2014 | pt_BR |
ISSN | 0074-0276 | |
URI | https://www.arca.fiocruz.br/handle/icict/9549 | |
Sponsorship | CNPq, LMW and MLB | pt_BR |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz | pt_BR |
Rights | open access | pt_BR |
Title | Mycobacteria mobility shift assay: a method for the rapid identification of Mycobacterium tuberculosis and nontuberculous mycobacteria | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/0074-0276130458. | |
Abstract | The identification of mycobacteria is essential because tuberculosis (TB) and mycobacteriosis are clinically indistinguishable and require different therapeutic regimens. The traditional phenotypic method is time consuming and may last up to 60 days. Indeed, rapid, affordable, specific and easy-to-perform identification methods are needed. We have previously described a polymerase chain reaction-based method called a mycobacteria mobility shift assay (MMSA) that was designed for Mycobacterium tuberculosis complex (MTC) and nontuberculous mycobacteria (NTM) species identification. The aim of this study was to assess the MMSA for the identification of MTC and NTM clinical isolates and to compare its performance with that of the PRA-hsp65 method. A total of 204 clinical isolates (102 NTM and 102 MTC) were identified by the MMSA and PRA-hsp65. For isolates for which these methods gave discordant results, definitive species identification was obtained by sequencing fragments of the 16S rRNA and hsp65 genes. Both methods correctly identified all MTC isolates. Among the NTM isolates, the MMSA alone assigned 94 (92.2%) to a complex or species, whereas the PRA-hsp65 method assigned 100% to a species. A 91.5% agreement was observed for the 94 NTM isolates identified by both methods. The MMSA provided correct identification for 96.8% of the NTM isolates compared with 94.7% for PRA-hsp65. The MMSA is a suitable auxiliary method for routine use for the rapid identification of mycobacteria | pt_BR |
Affilliation | Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis, SC, Brasil | pt_BR |
Affilliation | Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis, SC, Brasil | pt_BR |
Affilliation | Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis, SC, Brasil | pt_BR |
Affilliation | Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis, SC, Brasil | pt_BR |
Affilliation | Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis, SC, Brasil | pt_BR |
Affilliation | Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis, SC, Brasil | pt_BR |
Affilliation | Universidade Federal de Santa Catarina. Laboratório de Protozoologia. Florianópolis, SC, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Laboratório Central do Estado de Santa Catarina. Florianópolis, SC, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, Brasil | pt_BR |
Subject | nontuberculous mycobacteria | pt_BR |
Subject | mycobacteria mobility shift assay | pt_BR |
Subject | mycobacterial identification | pt_BR |