| Author | Masid-de-Brito, Daniela | |
| Author | Queto, Túlio | |
| Author | Gaspar-Elsas, Maria Ignez C. | |
| Author | Xavier-Elsas, Pedro Paulo | |
| Access date | 2015-03-04T14:51:10Z | |
| Available date | 2015-03-04T14:51:10Z | |
| Document date | 2014 | |
| Citation | MASID-DE-BRITO, Daniela. et al. Roles of 5-lipoxygenase and cysteinyl-leukotriene type 1 receptors in the hematological response to allergen challenge and its prevention by diethylcarbamazine in a murine model of asthma. Mediat. inflamm., New York, 2014. | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/9616 | |
| Language | eng | pt_BR |
| Publisher | Hindawi Publishing Corporation | pt_BR |
| Rights | open access | |
| Title | Roles of 5-lipoxygenase and cysteinyl-leukotriene type 1 receptors in the hematological response to allergen challenge and its prevention by diethylcarbamazine in a murine model of asthma | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1155/2014/403970 | |
| Abstract | Diethylcarbamazine (DEC), which blocks leukotriene production, abolishes the challenge-induced increase in eosinopoiesis
in bone-marrow from ovalbumin- (OVA-) sensitized mice, suggesting that 5-lipoxygenase (5-LO) products contribute to the
hematological responses in experimental asthma models. We explored the relationship between 5-LO, central and peripheral
eosinophilia, and effectiveness of DEC, using PAS or BALB/cmice and 5-LO-deficient mutants. We quantified eosinophil numbers
in freshly harvested or cultured bone-marrow, peritoneal lavage fluid, and spleen, with or without administration of leukotriene
generation inhibitors (DEC and MK886) and cisteinyl-leukotriene type I receptor antagonist (montelukast). The increase in
eosinophil numbers in bone-marrow, observed in sensitized/challenged wild-type mice, was abolished by MK886 and DEC
pretreatment. InALOXmutants, by contrast, therewas no increase inbone-marroweosinophil counts, nor ineosinophil production
in culture, in response to sensitization/challenge. In sensitized/challengedALOX mice, challenge-inducedmigration of eosinophils
to the peritoneal cavity was significantly reduced relative to the wild-type PAS controls. DEC was ineffective in ALOX mice, as
expected from a mechanism of action dependent on 5-LO. In BALB/c mice, challenge significantly increased spleen eosinophil
numbers andDECtreatment prevented this increase.Overall, 5-LOappears as indispensable to the systemichematological response
to allergen challenge, as well as to the effectiveness of DEC | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Saúde da Mulher, da Criança e do Adolescente Fernandes Figueira. Departamento de Pediatria. Rio de Janeito, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Saúde da Mulher, da Criança e do Adolescente Fernandes Figueira. Departamento de Pediatria. Rio de Janeito, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| DeCS | Araquidonato 5-Lipoxigenase | pt_BR |
| DeCS | Dietilcarbamazina | pt_BR |
| DeCS | Medula Óssea | pt_BR |
| DeCS | Eosinófilos | pt_BR |
| DeCS | Aloxano | pt_BR |
