| Author | Brunoro, Giselle Villa Flor | |
| Author | Carvalho, Paulo Costa | |
| Author | Ferreira, André Teixeira da Silva | |
| Author | Perales, Jonas | |
| Author | Valente, Richard Hemmi | |
| Author | Gallo, Claudia Vitória de Moura | |
| Author | Pagnoncelli, Dante | |
| Author | Neves-Ferreira, Ana Gisele da Costa | |
| Access date | 2015-03-17T14:45:53Z | |
| Available date | 2015-03-17T14:45:53Z | |
| Document date | 2015 | |
| Citation | BRUNORO, Giselle Villa Flor et al. Proteomic profiling of nipple aspirate fluid (NAF): exploring the complementarity of different peptide fractionation strategies. Journal of Proteomics, v. 117, p. 86–94, Mar. 2015. | pt_BR |
| ISSN | 1874-3919 | |
| URI | https://www.arca.fiocruz.br/handle/icict/9720 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Title | Proteomic profiling of nipple aspirate fluid (NAF): exploring the complementarity of different peptide fractionation strategies | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.jprot.2015.01.011 | |
| Abstract | NAF is a breast fluid that is closely related to the tumor microenvironment and a valuable sample for studying breast cancer. To performan in-depth proteomic analysis of this sample, aliquots of a single NAF digest were analyzed by the following peptide-centric fractionation strategies: a) 30-cm reversed-phase (RP) column on-line with an LTQ-Orbitrap XL; b) off-line strong cation-exchange (SCX) column; and c) pI-based OFFGEL fractionation. All fractions from approaches (b) and (c) were further analyzed on a 10-cm RP column hyphenated to the same mass spectrometer. The RP-30 cm, SCX/RP-10 cm, and OFFGEL/RP-10 cm approaches identified 1676, 2930, and 3240 peptides, which corresponded to 193, 390 and 528 proteins, respectively. In our cumulative dataset, 4466 distinct NAF peptides corresponded to a total of 557 proteins, of which only 34% were identified by all three approaches. No exclusive protein identification was associated to the RP-30 cm approach, while SCX/RP-10 cm and OFFGEL/ RP-10 cm contributed to 28 and 166 exclusive identifications, respectively. Each approach provided additional information related to energy metabolism (fermentation process/ carbohydrate biosynthesis). In conclusion, the pre-fractionation platforms used were complementary for the comprehensive characterization of NAF and our work provides methodological information for future quantitative cancer-related NAF sample studies. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Proteína Engenharia. Paraná, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Laboratório de Biologia Molecular de Tumores. Departamento de Genética. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Laboratório de Biologia Molecular Aplicada. Departamento de Ginecologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Pre-Fractionation | pt_BR |
| Subject | Shotgun | pt_BR |
| Subject | NAF | pt_BR |
| Subject | Breast Fluid | pt_BR |
| DeCS | Armas de Fogo | pt_BR |
| DeCS | Mama | pt_BR |
| Embargo date | 2030-12-31 | |
