| Author | Pereira, Lais | |
| Author | Abbehusen, Melissa Moura Costa | |
| Author | Teixeira, Clarissa Romero | |
| Author | Cunha, Jurema | |
| Author | Nascimento, Ivan Pereira | |
| Author | Fukutani, Kiyoshi Ferreira | |
| Author | dosSantos, Washington Luis Conrado | |
| Author | Barral, Aldina Maria Prado | |
| Author | Oliveira, Camila Indiani de | |
| Author | Barral Netto, Manoel | |
| Author | Soto, Manuel | |
| Author | Brodskyn, Claudia Ida | |
| Access date | 2015-03-17T16:58:44Z | |
| Available date | 2015-03-17T16:58:44Z | |
| Document date | 2015 | |
| Citation | PEREIRA, L. et al. Vaccination with Leishmania infantum Acidic Ribosomal P0 but Not with Nucleosomal Histones Proteins Controls Leishmania infantum Infection in Hamsters. PLoS Neglected Tropical Disese, v. 10, n. 2, p. e0003490, 2015. | pt_BR |
| ISSN | 1935-2735 | |
| URI | https://www.arca.fiocruz.br/handle/icict/9725 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Leishmania infantum | pt_BR |
| Subject in Portuguese | Vacinação | pt_BR |
| Subject in Portuguese | Ratos | pt_BR |
| Subject in Portuguese | Proteínas | pt_BR |
| Subject in Portuguese | Infecção | pt_BR |
| Subject in Portuguese | Ácido | pt_BR |
| Title | Vaccination with Leishmania infantum Acidic Ribosomal P0 but Not with Nucleosomal Histones Proteins Controls Leishmania infantum Infection in Hamsters. | pt_BR |
| Type | Article | pt_BR |
| DOI | :10.1371/journal.pntd.0003490 | |
| Abstract | BACKGROUND: Several intracellular Leishmania antigens have been identified in order to find a potential vaccine capable of conferring long lasting protection against Leishmania infection. Histones and Acid Ribosomal proteins are already known to induce an effective immune response and have successfully been tested in the cutaneous leishmaniasis mouse model. Here, we investigate the protective ability of L. infantum nucleosomal histones (HIS) and ribosomal acidic protein P0 (LiP0) against L. infantum infection in the hamster model of visceral leishmaniasis using two different strategies: homologous (plasmid DNA only) or heterologous immunization (plasmid DNA plus recombinant protein and adjuvant). METHODOLOGY/PRINCIPAL FINDINGS: Immunization with both antigens using the heterologous strategy presented a high antibody production level while the homologous strategy immunized group showed predominantly a cellular immune response with parasite load reduction. The pcDNA-LiP0 immunized group showed increased expression ratio of IFN-γ/IL-10 and IFN-γ/TGF-ß in the lymph nodes before challenge. Two months after infection hamsters immunized with the empty plasmid presented a pro-inflammatory immune response in the early stages of infection with increased expression ratio of IFN-γ/IL-10 and IFN-γ/TGF-ß, whereas hamsters immunized with pcDNA-HIS presented an increase only in the ratio IFN-γ/ TGF-ß. On the other hand, hamsters immunized with LiP0 did not present any increase in the IFN-γ/TGF-ß and IFN-γ/IL-10 ratio independently of the immunization strategy used. Conversely, five months after infection, hamsters immunized with HIS maintained a pro-inflammatory immune response (ratio IFN-γ/ IL-10) while pcDNA-LiP0 immunized hamsters continued showing a balanced cytokine profile of pro and anti-inflammatory cytokines. Moreover we observed a significant reduction in parasite load in the spleen, liver and lymph node in this group compared with controls. CONCLUSIONS/SIGNIFICANCE: Our results suggest that vaccination with L. infantum LiP0 antigen administered in a DNA formulation could be considered a potential component in a vaccine formulation against visceral leishmaniasis. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Instituto Butantan. Centro de Biotecnologia. São Paulo, SP, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, Brasil | pt_BR |
| Affilliation | Universidad Autónoma de Madrid. Centro de Biología Molecular "Severo Ochoa." Madrid, Spain | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil | pt_BR |
