| Author | Silva, Rafael Paletta | |
| Author | Vieira, Danielle P | |
| Author | Berbardo, Rodrigo Vieira | |
| Author | Majerowicz, David | |
| Author | Gondim, Katia Calp | |
| Author | Santos, Marcos André Vannier dos | |
| Author | Lopes, Angela Hampshire de Carvalho Santos | |
| Author | Fernandes, José Roberto Meyer | |
| Access date | 2015-03-31T16:27:40Z | |
| Available date | 2015-03-31T16:27:40Z | |
| Document date | 2011 | |
| Citation | PALETTA-SILVA, R. et al. Leishmania amazonensis: characterization of an ecto-3'-nucleotidase activity and its possible role in virulence. Experimental Parasitology, v. 129, n. 3, p.277-283,, 2011. | pt_BR |
| ISSN | 0014-4894 | |
| URI | https://www.arca.fiocruz.br/handle/icict/9867 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | pt_BR |
| Title | Leishmania amazonensis: characterization of an ecto-3´-nucleotidase activity and its possible role in virulence | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.exppara.2011.07.014 | |
| Abstract | Ecto-30-nucleotidase/nuclease (30NT/NU) is a membrane-bound enzyme that plays a key role in the nutrition
of Leishmania sp. protozoan parasites. This enzyme generates nucleosides via hydrolyzes of 30mononucleotides
and nucleic acids, which enter the cell by specific transporters. In this work, we identify and
characterize Leishmania amazonensis ecto-30-nucleotidase activity (La30-nucleotidase), report ammonium
tetrathiomolybdate (TTM) as a novel La30-nucleotidase inhibitor and approach the possible involvement
of ecto-30-nucleotidase in cellular adhesion. La30-nucleotidase presented characteristics similar to those
reported for the class I single-strand nuclease family; a molecular weight of approximately 40 kDa and
optimum activity in an alkaline pH range were observed. Although it is conserved among the genus,
La30-nucleotidase displays different kinetic properties; it can be inhibited by vanadate, molybdate and
Cu2+ ions. Interestingly, ecto-30-nucleotidase activity is 60-fold higher than that of ecto-50-nucleotidase
in L. amazonensis. Additionally, ecto-30-nucleotidase activity is two-fold higher in virulent L. amazonensis
cells than in avirulent ones. Notably, macrophage–parasite attachment/invasion was increased by 400%
in the presence of adenosine 30-monophosphate (30AMP); however, this effect was reverted by TTM treatment.
We believe that La30-nucleotidase may play a significant role in the generation of adenosine, which
may contribute to mammalian host immune response impairment and establishment of infection | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil | pt_BR |
| Subject | Ecto-30-nucleotidase | pt_BR |
| Subject | Ecto-50-nucleotidase | pt_BR |
| Subject | Leishmania amazonensis | pt_BR |
| Subject | Cellular adhesion | pt_BR |
| Subject | Adenosine | pt_BR |
| Subject | Ammonium tetrathiomolybdate | pt_BR |
| DeCS | Leishmania mexicana/enzimologia | pt_BR |
| DeCS | Leishmania mexicana/patogenicidade | pt_BR |
| DeCS | Macrófagos Peritoneais/parasitologia | pt_BR |
| DeCS | Nucleotidases/metabolismo | pt_BR |
| DeCS | Monofosfato de Adenosina | pt_BR |
| DeCS | Virulencia | pt_BR |
