Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10076
Title: Reevaluating the Trypanosoma cruzi proteomic map: The shotgun description of bloodstream trypomastigotes
Authors: Brunoro, Giselle Villa Flor
Caminha, Marcelle Almeida
Ferreira, André Teixeirada da Silva
Leprevost, Felipeda da Veiga
Carvalho, Paulo Costa
Perales, Jonas
Valente, Richard Hemmi
Menna-Barreto, Rubem Figueiredo Sadok
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
undação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Paraná, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Paraná, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Abstract: Chagas disease is a neglected disease, caused by the protozoan Trypanosoma cruzi. This kinetoplastid presents a cycle involving different forms and hosts, being trypomastigotes the main infective form. Despite various T. cruzi proteomic studies, the assessment of bloodstream trypomastigote profile remains unexplored. The aim of this work is T. cruzi bloodstream form proteomic description. Employing shotgun approach, 17,394 peptides were identified, corresponding to 7514 proteins of which 5901 belong to T. cruzi. Cytoskeletal proteins, chaperones, bioenergetics-related enzymes, and trans-sialidases are among the top-scoring. GO analysis revealed that all T. cruzi compartments were assessed; and majority of proteins are involved in metabolic processes and/or presented catalytic activity. The comparative analysis between the bloodstream trypomastigotes and cultured-derived or metacyclic trypomastigote proteomic profiles pointed to 2202 proteins exclusively detected in the bloodstream form. These exclusive proteins are related to: (a) surface proteins; (b) non-classical secretion pathway; (c) cytoskeletal dynamics; (d) cell cycle and transcription; (e) proteolysis; (f) redoxmetabolism; (g) biosynthetic pathways; (h) bioenergetics; (i) protein folding; (j) cell signaling; (k) vesicular traffic; (l) DNA repair; and (m) cell death. This large-scale evaluation of bloodstream trypomastigotes, responsible for the parasite dissemination in the patient, marks a step forward in the comprehension of Chagas disease pathogenesis.
Keywords: Trypanosoma cruzi
Bloodstream trypomastigotes
Proteomics
Mass spectrometry
Issue Date: 2015
Publisher: Elsevier
Citation: BRUNORO, G. V. et al.Reevaluating the Trypanosoma cruzi proteomic map: The shotgun description of bloodstream trypomastigotes. Journal of Proteomics, n.115, p. 58-65, 2015.
DOI: 10.1016/j.jprot.2014.12.003
Embargo date: Feb. 2016
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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