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INTRANASAL VACCINATION WITH EXTRACELLULAR SERINE PROTEASES OF LEISHMANIA AMAZONENSIS CONFERS PROTECTIVE IMMUNITY TO BALB/C MICE AGAINST INFECTION
Murine cutaneous leishmaniasis
Mucosal vaccine
Intranasal route
Extracellular serine proteases
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Abstract
Background: Previously, we demonstrated that unlike subcutaneous or intramuscular vaccination, intranasal
vaccination of BALB/c mice with whole Leishmania amazonensis antigens leads to protection against cutaneous
leishmaniasis. Here, the role of parasite serine proteases in the protective immunity was investigated.
Findings: Serine Proteases were partially purified from both soluble (LaSP-Sol) and extracellular (LaSP-Ex) Leishmania
amazonensis promastigote extracts by aprotinin-agarose chromatography. BALB/c mice were intranasally immunized
with LaSP-Sol and LaSP-Ex prior to infection with L. amazonensis. LaSP-Ex but not LaSP-Sol vaccination led to
significantly smaller lesions and parasite burdens as compared with non-vaccinated controls. Protection was
accompanied by systemic Th1 polarization with increased IFN-γ and decreased IL-4 and IL-10 splenic production.
Likewise, increased production of IFN-γ, IL-12 and IL-4 concomitant with decreased TGF-β and TNF-α was locally
observed in the infected footpad.
Conclusion: This study indicates that extracellular serine proteases of L. amazonensis are strong candidates for a
more defined intranasal vaccine against cutaneous leishmaniasis.
Keywords
Leishmania amazonensisMurine cutaneous leishmaniasis
Mucosal vaccine
Intranasal route
Extracellular serine proteases
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