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Sustainable Development Goals
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- IOC - Artigos de Periódicos [12969]
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EFFECTS OF CHOLINERGIC STIMULATION WITH PYRIDOSTIGMINE BROMIDE ON CHRONIC CHAGASIC CARDIOMYOPATHIC MICE
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Abstract
The aim of the present study was to assess the effects of an anticholinesterase agent, pyridostigmine bromide (Pyrido), on
experimental chronic Chagas heart disease in mice. To this end, male C57BL/6J mice noninfected (control:Con) or chronically
infected (5 months) with Trypanosoma cruzi (chagasic:Chg) were treated or not (NT) with Pyrido for one month. At the end
of this period, electrocardiogram (ECG); cardiac autonomic function; heart histopathology; serum cytokines; and the presence
of blood and tissue parasites by means of immunohistochemistry and PCR were assessed. In NT-Chg mice, significant changes
in the electrocardiographic, autonomic, and cardiac histopathological profiles were observed confirming a chronic inflammatory
response. Treatment with Pyrido in Chagasic mice caused a significant reduction of myocardial inflammatory infiltration, fibrosis,
and hypertrophy, which was accompanied by a decrease in serum levels of IFN𝛾 with no change in IL-10 levels, suggesting a shift of
immune response toward an anti-inflammatory profile. Lower nondifferent numbers of parasite DNA copies were observed in both
treated and nontreated chagasic mice. In conclusion, our findings confirmthe marked neuroimmunomodulatory role played by the
parasympathetic autonomic nervous systemin the evolution of the inflammatory-immune response to T. cruzi during experimental
chronic Chagas heart disease in mice.
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