Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10209
Title: Multidrug Resistant Mycobacterium tuberculosis: A Retrospective katG and rpoB Mutation Profile Analysis in Isolates from a Reference Center in Brazil
Authors: Freitas, Flávia A. D. de
Bernardo, Vagner
Gomgnimbou, Michel K
Sola, Christophe
Siqueira, Hélio R
Pereira, Márcia A. S
Fandinho, Fátima C. O
Gomes, Harrison M
Araújo, Marcelo E. I
Suffys, Philip Noel
Marques, Elizabeth A
Albano, Rodolpho Mattos
Affilliation: Universidade do Estado do Rio de Janeiro (UERJ). Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro (UERJ). Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.
CNRS–Université Paris–Sud, Institut de Génétique et Microbiologie– Infection Genetics Emerging Pathogens Evolution Team. Orsay, France.
CNRS–Université Paris–Sud, Institut de Génétique et Microbiologie– Infection Genetics Emerging Pathogens Evolution Team. Orsay, France.
Universidade do Estado do Rio de Janeiro (UERJ). Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Centro de Referência Professor Hélio Fraga. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Centro de Referência Professor Hélio Fraga. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro (UERJ). Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro (UERJ). Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.
Abstract: Background: Multidrug resistance is a critical factor in tuberculosis control. To gain better understanding of multidrug resistant tuberculosis in Brazil, a retrospective study was performed to compare genotypic diversity and drug resistance associated mutations in Mycobacterium tuberculosis isolates from a national reference center. Methods and Findings: Ninety-nine multidrug resistant isolates from 12 Brazilian states were studied. Drug-resistance patterns were determined and the rpoB and katG genes were screened for mutations. Genotypic diversity was investigated by IS6110-RFLP and Luminex 47 spoligotyping. Mutations in rpoB and katG were seen in 91% and 93% of the isolates, respectively. Codon 315 katG mutations occurred in 82.8% of the isolates with a predominance of the Ser315Thr substitution. Twenty-five isolates were clustered in 11 groups with identical IS6110-RFLP patterns while 74 showed unique patterns with no association between mutation frequencies or susceptibility profiles. The most prevalent spoligotyping lineages were LAM (47%), T (17%) and Haarlen (12%). The Haarlen lineage showed a higher frequency of codon 516 rpoB mutations while codon 531 mutations prevailed in the other isolates. Conclusions: Our data suggest that there were no major multidrug resistant M. tuberculosis strains transmitted among patients referred to the reference center, indicating an independent acquisition of resistance. In addition, drug resistance associated mutation profiles were well established among the main spoligotyping lineages found in these Brazilian multidrug resistant isolates, providing useful data for patient management and treatment.
Keywords: Mycobacterium tuberculosis
Brazil
Reference Center
Keywords in spanish: Tuberculosis
keywords: Tuberculose
Brasil
Issue Date: 2014
Publisher: Plos One
Citation: FREITAS. Flávia A. D. de et al. Multidrug Resistant Mycobacterium tuberculosis: A Retrospective katG and rpoB Mutation Profile Analysis in Isolates from a Reference Center in Brazil. Plos One, v.9, n.8, 11p, 2014.
DOI: 10.1371/journal.pone.0104100
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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