Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10221
Title: Aflagellar epimastigote forms are found in axenic culture ofTrypanosoma caninum
Authors: Barros, Juliana H. S
Fonseca, Tatiana S
Macedo-Silva, Roger M
Côrte-Real, Suzana
Toma, Helena K
Madeira, Maria de Fátima
Affilliation: Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Programa de Pós-Graduação em Pesquisa Clínica em Doenças Infecciosas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma Multiusuários Rudolf Barth. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Faculdade de Farmácia. Laboratório de Diagnóstico Molecular e Hematologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.
Abstract: tRepresentatives of the genus Trypanosoma have been traditionally found in epimastigote, espheromastig-ote and trypomastigote flagellated forms in axenic cultures. Trypanosoma caninum is a trypanosomatidthat has recently been reported infecting dogs in endemic areas of canine leishmaniasis in Brazil. Itpresents specific biological characteristics and it is found exclusively on healthy skin. Here, we describethe evolutive forms of this parasite showing not only the forms commonly found in culture, but alsoepimastigote forms with no free flagellum. The study was conducted using scanning and transmissionelectron microscopy and, we demonstrate that typical flagellated epimastigotes originate from formswithout flagellum, although the latter may remain without differentiation in the culture. Two hypothesesare considered and discussed in this paper: (i) the aflagellated epimastigotes are a typical developmentalforms of T. caninum and (ii) the emergence of these aflagellated forms could be resultant from a disturbedprocess during cell division caused by interfering specific proteins, which leads to inability to form andregulate the flagellum length. In any case, considering that T. caninum is a parasite that is still little stud-ied, the information brought by our study adds data which may be useful to clarify aspects on the cellcycle of this intriguing parasite that has been found in different regions of Brazil.
Keywords: Trypanosomatidae
Dog
Ultrastructure
Electron microscopy
Light microscopy
Keywords in spanish: Enfermedades de los Perros
Trypanosomatina
Microscopía Electrónica
keywords: Doenças do Cão
Trypanosomatina
Microscopia Eletrônica
Issue Date: 2014
Publisher: Elsevier
Citation: BARROS, Juliana H. S et al. Aflagellar epimastigote forms are found in axenic culture ofTrypanosoma caninum. Acta Tropica, n, 137, p.147-151, 2014.
DOI: 10.1016/j.actatropica.2014.05.013
ISSN: 0001-706X
Copyright: restricted access
Appears in Collections:INI - Artigos de Periódicos
IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
suzana_corterealetal_IOC_2014.pdf2.4 MBAdobe PDF    Request a copy


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.