| Author | Narciso, Juliana Oliveira Abreu | |
| Author | Soares, Renata Oliveira de Araújo | |
| Author | Santos-Mallet, Jacenir R. | |
| Author | Guimarães, Anthony Érico | |
| Author | Chaves, Maria Célia de Oliveira | |
| Author | Barbosa Filho, José Maria | |
| Author | Maleck, Marise | |
| Access date | 2015-05-15T13:16:48Z | |
| Available date | 2015-05-15T13:16:48Z | |
| Document date | 2014 | |
| Citation | NARCISO, Juliana Oliveira Abreu et al. Burchellin: study of bioactivity against Aedes aegypti. Parasites & Vectors, v.7, n.172, 10p, 2014. | pt_BR |
| ISSN | 1756-3305 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/10351 | |
| Language | eng | pt_BR |
| Publisher | BioMed Central | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Dengue | pt_BR |
| Subject in Portuguese | Aedes | pt_BR |
| Title | Burchellin: study of bioactivity against Aedes aegypti | pt_BR |
| Type | Article | |
| DOI | 10.1186/1756-3305-7-172 | pt_BR |
| Abstract | Background: The dengue mosquito Aedes aegypti Linnaeus, 1762 is a widespread insect pest of serious medical
importance. Since no effective vaccine is available for treating dengue, the eradication or control of the main
mosquito vector is regarded as essential. Since conventional insecticides have limited success, plants may be an
alternative source of larvicidal agents, since they contain a rich source of bioactive chemicals. The aim of this study
was to evaluate the larvicidal activity of the neolignan burchellin isolated from Ocotea cymbarum (Lauraceae), a
plant from the Amazon region, against third instar larvae of A. aegypti.
Methods: Burchellin obtained from O. cymbarum was analyzed. The inhibitory activity against A. aegypti eggs and
larvae and histological changes in the digestive system of treated L3 larvae were evaluated. In addition, nitric oxide
synthase activity and nitric oxide levels were determined, and cytotoxicity bioassays performed.
Results: The data showed that burchellin interfered with the development cycle of the mosquito, where its strongest
toxic effect was 100% mortality in larvae (L3) at concentrations ≥ 30 ppm. This compound did not show target cell
toxicity in peritoneal macrophages from BALB/c mice, and proved to have molecular stability when dissolved in water.
The L3 and L4 larvae treated with the compound showed cellular destruction and disorganization, cell spacing, and
vacuolization of epithelial cells in small regions of the midgut.
Conclusion: The neolignan burchellin proved to be a strong candidate for a natural, safe and stable phytolarvicidal to
be used in population control of A. aegypti. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Díptera. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Setor de Entomologia Médica e Forense. Laboratório de Transmissores de Leishmanioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Díptera. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Paraíba. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Paraíba. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Díptera. Rio de Janeiro, RJ, Brasil / Universidade Severino Sombra. Laboratório de Insetos Vetores, Ciências da saúde. Mestrado Profissional em Ciências Ambientais. Vassouras, RJ, Brasil. | pt_BR |
| Subject | Dengue | pt_BR |
| Subject | Aedes aegypti (L.) | pt_BR |
| Subject | Neolignan burchellin | pt_BR |
| Subject | Ocotea cymbarum (Lauraceae) | pt_BR |
| Subject | Larvicidal activity | pt_BR |
| Subject in Spanish | Dengue | pt_BR |
| Subject in Spanish | Aedes | pt_BR |
| DeCS | Aedes | pt_BR |
