Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10351
Title: Burchellin: study of bioactivity against Aedes aegypti
Authors: Narciso, Juliana Oliveira Abreu
Soares, Renata Oliveira de Araújo
Santos-Mallet, Jacenir R.
Guimarães, Anthony Érico
Chaves, Maria Célia de Oliveira
Barbosa Filho, José Maria
Maleck, Marise
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Díptera. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Setor de Entomologia Médica e Forense. Laboratório de Transmissores de Leishmanioses. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Díptera. Rio de Janeiro, RJ, Brasil.
Universidade Federal da Paraíba. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, Brasil.
Universidade Federal da Paraíba. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Díptera. Rio de Janeiro, RJ, Brasil / Universidade Severino Sombra. Laboratório de Insetos Vetores, Ciências da saúde. Mestrado Profissional em Ciências Ambientais. Vassouras, RJ, Brasil.
Abstract: Background: The dengue mosquito Aedes aegypti Linnaeus, 1762 is a widespread insect pest of serious medical importance. Since no effective vaccine is available for treating dengue, the eradication or control of the main mosquito vector is regarded as essential. Since conventional insecticides have limited success, plants may be an alternative source of larvicidal agents, since they contain a rich source of bioactive chemicals. The aim of this study was to evaluate the larvicidal activity of the neolignan burchellin isolated from Ocotea cymbarum (Lauraceae), a plant from the Amazon region, against third instar larvae of A. aegypti. Methods: Burchellin obtained from O. cymbarum was analyzed. The inhibitory activity against A. aegypti eggs and larvae and histological changes in the digestive system of treated L3 larvae were evaluated. In addition, nitric oxide synthase activity and nitric oxide levels were determined, and cytotoxicity bioassays performed. Results: The data showed that burchellin interfered with the development cycle of the mosquito, where its strongest toxic effect was 100% mortality in larvae (L3) at concentrations ≥ 30 ppm. This compound did not show target cell toxicity in peritoneal macrophages from BALB/c mice, and proved to have molecular stability when dissolved in water. The L3 and L4 larvae treated with the compound showed cellular destruction and disorganization, cell spacing, and vacuolization of epithelial cells in small regions of the midgut. Conclusion: The neolignan burchellin proved to be a strong candidate for a natural, safe and stable phytolarvicidal to be used in population control of A. aegypti.
Keywords: Dengue
Aedes aegypti (L.)
Neolignan burchellin
Ocotea cymbarum (Lauraceae)
Larvicidal activity
Keywords in spanish: Dengue
Aedes
keywords: Dengue
Aedes
DeCS: Aedes
Issue Date: 2014
Publisher: BioMed Central
Citation: NARCISO, Juliana Oliveira Abreu et al. Burchellin: study of bioactivity against Aedes aegypti. Parasites & Vectors, v.7, n.172, 10p, 2014.
DOI: 10.1186/1756-3305-7-172
ISSN: 1756-3305
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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