Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10396
Title: Apoptotic Mediators in Patients With Severe and Non-Severe Dengue From Brazil
Authors: Limonta, Daniel
Carvalho, Amanda Torrentes
Marinho, Cíntia Ferreira
Azeredo, Elzinandes Leal de
Souza, Luiz José de
Castro, Ana Rita C. Motta
Cunha, Rivaldo Venâncio
Kubelka, Claire Fernandes
Nogueira, Rita Maria Ribeiro
Pinto, Luzia Maria de Oliveira
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Laboratório de Flavivirus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.
Centro Regional de Referência em Dengue. Campos dos Goytacazes, Rio de Janeiro, RJ, Brasil.
Universidade Federal do Mato Grosso. Setor Hemonúcleo. MS, Brasil.
Universidade Federal do Mato Grosso. Setor Hemonúcleo. MS, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivirus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.
Abstract: Despite being the most significant arboviral disease worldwide, dengue has no antiviral treatment or reliable severity predictors. It has been shown that apoptotic cells from blood and tissues may be involved in the complex pathogenesis of dengue. However, very little is known about the interplay between proapoptotic and antiapoptotic mediators in this disease. Therefore, plasma levels of the three proapoptotic mediators Fas ligand (FasL), tumor necrosis factor-α (TNF-α), and TNF-related apoptosis-inducing ligand (TRAIL) were measured in dengue patients. Patients were classified according to the World Health Organization classification of dengue revised in 2009. Additionally, inhibitors of apoptosis protein (IAPs) were determined in plasma (Survivin) and peripheral blood mononuclear cells (PBMCs) lysates (cIAP-1, cIAP-2, XIAP). Levels of apoptotic proteins in plasma were correlated with counts of blood cells. FasL and TRAIL levels were elevated in dengue patients without warning signs when compared to patients with severe dengue and controls. Dengue patients with warning signs showed decreased levels of Survivin compared to patients with severe dengue and controls. TRAIL was inversely correlated with counts of lymphocyte subsets. In contrast, Survivin was positively correlated with leukocyte counts. There was a trend of elevated IAPs levels in PBMCs of patients with severe dengue. The results suggest a likely antiviral effect of TRAIL in dengue. It appears that TRAIL might be involved with apoptosis induction of lymphocytes, whereas IAPs might participate in protecting leukocytes from apoptosis. Further research is needed to explore the interactions between pro and antiapoptotic molecules and their implications in dengue pathogenesis.
Keywords: Apoptosis
Pathogenesis
Phocytes
Monocytes
keywords: Apoptose
Patogênese
Monócitos
DeCS: Apoptose
Monócitos
Issue Date: 2014
Publisher: Wiley Periodicals, INC
Citation: LIMONTA, Daniel et al. Apoptotic Mediators in Patients With Severe and Non-Severe Dengue From Brazil. Journal of Medical Virology, v.86, n.8, p.1437–1447, 2014.
DOI: 10.1002/jmv.23832
ISSN: 1096-9071
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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