Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10434
Title: Detection of parasite antigens in Leishmania infantum-infected spleen tissue by monoclonal antibody-, piezoelectric-based immunosensors.
Authors: Miranda, Gustavo Cabral
Jesus, Joilson Ramos de
Oliveira, Pablo Rafael Silveira
Britto, G. S. G
Pontes-de-Carvalho, Lain Carlos
Dutra, R. F
Neves, Neuza Maria Alcântara
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Universidade Federal de Pernambuco. Laboratório de EngenhariaBiomédica. Recife, PE, Brasil
Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeração. Salvador, BA, Brasil
Abstract: Diseases such as leishmaniases are important causes of morbidity and mortality in Brazil, and their diagnoses need to be improved. The use of monoclonal antibodies has ensured high specificity to immunodiagnosis. The development of an immunosensor, coupling a monoclonal antibody to a bioelectronic device capable of quickly detecting Leishmania sp. antigens both qualitatively and quantitatively, is a promising alternative for the diagnosis of leishmaniasis due to its high specificity, low cost, and portability, compared with conventional methods. The present work was aimed at developing an immunosensor-based assay for detecting Leishmania infantum antigens in tissues of infected hosts. Four hybridomas producing monoclonal antibodies against L. infantum had their specificity confirmed by enzyme-linked immunosorbent assay. These antibodies were immobilized on a gold surface, covered with a thin film of 2-aminoethanethiol (cysteamine) and glutaraldehyde, blocked with glycine, and placed into contact with extracts of L. infantum -infected and noninfected control hamster spleens. The assay was able to detect 1.8 × 10(4) amastigotes/g of infected tissue. These results demonstrated that this assay may be useful for quantifying L. infantum amastigotes in organs of experimental animals for studies on pathogenesis and immunity and that it is a promising tool for the development of a diagnostic method, based on antigen detection, of human and dog visceral leishmaniasis.
DeCS: Anticorpos Monoclonais/uso diagnóstico
Antígenos de Protozoários/isolamento & purificação
Leishmania infantum/imunologia
Leishmaniose Visceral/diagnóstico
Baço/parasitologia
Animais
Anticorpos Monoclonais/imunologia
Anticorpos Antiprotozoários/uso diagnóstico
Especificidade de Anticorpos
Técnicas Biossensoriais/métodos
Cricetinae
Ensaio de Imunoadsorção Enzimática
Imunoglobulina G/uso diagnóstico
Leishmania infantum/isolamento & purificação
Mesocricetus
Camundongos
Camundongos Endogâmicos BALB C
Organismos Livres de Patógenos Específicos
Baço/imunologia
Issue Date: 2014
Publisher: American Society of Parasitologists
Citation: MIRANDA, G. C. et al. Detection of parasite antigens in Leishmania infantum-infected spleen tissue by monoclonal antibody-, piezoelectric-based immunosensors. Journal of Parasitology, v. 100, n. 1, p. 73-78, 2014.
DOI: dx.doi.org/10.1645/GE-3052.1
ISSN: 1937-2345
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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