Mostrar el registro sencillo del ítem

AutorLeal, Marcos Maurício Tosta
AutorFerro, Zaquer Suzana Munhoz Costa
AutorSouza, Bruno Solano de Freitas
AutorAzevedo, Carine Machado
AutorCarvalho, Thiago Meneses
AutorKaneto, Carla Martins
AutorCarvalho, Rejane Hughes
AutorSantos, Ricardo Ribeiro dos
AutorSoares, Milena Botelho Pereira
Fecha de acceso2015-05-19T17:50:20Z
Fecha de disponibilización2015-05-19T17:50:20Z
Fecha de publicación2014
ReferenciaLEAL, M. M. T. et al. Early transplantation of bone marrow mononuclear cells promotes neuroprotection and modulation of inflammation after status epilepticus in mice by paracrine mechanisms. Neurochemical Research, v. 39, n. 2, p. 259-268, 2014.pt_BR
ISSN1573-6903
ISSN10.1007/s11064-013-1217-7
URIhttps://www.arca.fiocruz.br/handle/icict/10435
Idiomaengpt_BR
EditorSpringer Verlagpt_BR
Derechos de autoropen accesspt_BR
TítuloEarly transplantation of bone marrow mononuclear cells promotes neuroprotection and modulation of inflammation after status epilepticus in mice by paracrine mechanisms.pt_BR
Tipo del documentoArticlept_BR
Resumen en InglésStatus epilepticus (SE) is a severe clinical manifestation of epilepsy associated with intense neuronal loss and inflammation, two key factors involved in the pathophysiology of temporal lobe epilepsy. Bone marrow mononuclear cells (BMMC) attenuated the consequences of pilocarpine-induced SE, including neuronal loss, in addition to frequency and duration of seizures. Here we investigated the effects of BMMC transplanted early after the onset of SE in mice, as well as the involvement of soluble factors produced by BMMC in the effects of the cell therapy. Mice were injected with pilocarpine for SE induction and randomized into three groups: transplanted intravenously with 1 × 10(7) BMMC isolated from GFP transgenic mice, injected with BMMC lysate, and saline-treated controls. Cell tracking, neuronal counting in hippocampal subfields and cytokine analysis in the serum and brain were performed. BMMC were found in the brain 4 h following transplantation and their numbers progressively decreased until 24 h following transplantation. A reduction in hippocampal neuronal loss after SE was found in mice treated with live BMMC and BMMC lysate when compared to saline-treated, SE-induced mice. Moreover, the expression of inflammatory cytokines IL-1ß, TNF-α, IL-6 was decreased after injection of live BMMC and to a lesser extent, of BMMC lysate, when compared to SE-induced controls. In contrast, IL-10 expression was increased. Analysis of markers for microglia activation demonstrated a reduction of the expression of genes related to type 1-activation. BMMC transplantation promotes neuroprotection and mediates anti-inflammatory effects following SE in mice, possibly through the secretion of soluble factors.pt_BR
AfiliaciónHospital São Rafael. Salvador, BA, Brasilpt_BR
AfiliaciónHospital São Rafael. Salvador, BA, Brasilpt_BR
AfiliaciónHospital São Rafael. Salvador, BA, Brasilpt_BR
AfiliaciónHospital São Rafael. Salvador, BA, Brasilpt_BR
AfiliaciónHospital São Rafael. Salvador, BA, Brasilpt_BR
AfiliaciónHospital São Rafael. Salvador, BA, Brasilpt_BR
AfiliaciónHospital São Rafael. Salvador, BA, Brasilpt_BR
AfiliaciónHospital São Rafael. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasilpt_BR
AfiliaciónHospital São Rafael. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasilpt_BR
DeCSTransplante de Medula Ósseapt_BR
DeCSFármacos Neuroprotetorespt_BR
DeCSPilocarpina/administração & dosagempt_BR
DeCSEstado Epiléptico/induzido quimicamentept_BR
DeCSAnimaispt_BR
DeCSSequência de Basespt_BR
DeCSCitocinas/biossíntesept_BR
DeCSPrimers do DNApt_BR
DeCSExpressão Gênicapt_BR
DeCSProteínas de Fluorescência Verde/genéticapt_BR
DeCSCamundongospt_BR
DeCSCamundongos Endogâmicos C57BLpt_BR
DeCSCamundongos Transgênicospt_BR
DeCSMicroglia/metabolismopt_BR
DeCSReação em Cadeia da Polimerase Via Transcriptase Reversapt_BR
DeCSEstado Epiléptico/cirurgiapt_BR


Ficheros en el ítem

application/pdf
Nombre:
Leal MMT Early....pdf
Tamaño:
763.2Kb
Formato:
application/
Ver/Abrir

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem